Journal article
Saccharomyces cerevisiae Mer3 Helicase Stimulates 3′–5′ Heteroduplex Extension by Rad51 : Implications for Crossover Control in Meiotic Recombination
Cell (Cambridge), v 117(1)
2004
PMID: 15066281
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Crossover and noncrossover recombinants can form by two different pathways during meiotic recombination in
Saccharomyces cerevisiae. The
MER3 gene is known to affect selectively crossover, but not noncrossover, recombination. The Mer3 protein is a DNA helicase that unwinds duplex DNA in the 3′ to 5′ direction. To define the underlying molecular steps of meiotic recombination, we investigated the role of Mer3 helicase in DNA strand exchange promoted by Rad51 protein. We found that Mer3 helicase does not function as an initiator of DNA pairing events but, rather, it stimulates DNA heteroduplex extension in the 3′ → 5′ direction relative to the incoming (or displaced) single-stranded DNA. Conversely, Mer3 helicase blocks DNA heteroduplex extension in the 5′ → 3′ direction. Our results support the idea that Mer3 helicase stabilizes nascent joint molecules via DNA heteroduplex extension to permit capture of the second processed end of a double-stranded DNA break, a step which is required for crossover recombinant product formation.
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Details
- Title
- Saccharomyces cerevisiae Mer3 Helicase Stimulates 3′–5′ Heteroduplex Extension by Rad51 : Implications for Crossover Control in Meiotic Recombination
- Creators
- Olga M Mazina - Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, CA 95616 USAAlexander V Mazin - Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, CA 95616 USATakuro Nakagawa - Ludwig Institute for Cancer Research, Cancer Center and Department of Medicine, University of California San Diego School of Medicine, La Jolla, CA 92093 USARichard D Kolodner - Ludwig Institute for Cancer Research, Cancer Center and Department of Medicine, University of California San Diego School of Medicine, La Jolla, CA 92093 USAStephen C Kowalczykowski - Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, CA 95616 USA
- Publication Details
- Cell (Cambridge), v 117(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000221500300007
- Scopus ID
- 2-s2.0-1842816528
- Other Identifier
- 991014878240404721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology