Journal article
Salmonella and Escherichia coli O157:H7 Survival in Soil and Translocation into Leeks (Allium porrum) as Influenced by an Arbuscular Mycorrhizal Fungus (Glomus intraradices)
Applied and environmental microbiology, v 79(6), pp 1813-1820
01 Mar 2013
PMID: 23315740
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A study was conducted to determine the influence of arbuscular mycorrhizal (AM) fungi on
Salmonella
and enterohemorrhagic
Escherichia coli
O157:H7 (EHEC) in autoclaved soil and translocation into leek plants. Six-week-old leek plants (with [Myc+] or without [Myc−] AM fungi) were inoculated with composite suspensions of
Salmonella
or EHEC at ca. 8.2 log CFU/plant into soil. Soil, root, and shoot samples were analyzed for pathogens on days 1, 8, 15, and 22 postinoculation. Initial populations (day 1) were ca. 3.1 and 2.1 log CFU/root, ca. 2.0 and 1.5 log CFU/shoot, and ca. 5.5 and 5.1 CFU/g of soil for
Salmonella
and EHEC, respectively. Enrichments indicated that at days 8 and 22, only 31% of root samples were positive for EHEC, versus 73% positive for
Salmonella
. The mean
Salmonella
level in soil was 3.4 log CFU/g at day 22, while EHEC populations dropped to ≤0.75 log CFU/g by day 15. Overall,
Salmonella
survived in a greater number of shoot, root, and soil samples, compared with the survival of EHEC. EHEC was not present in Myc− shoots after day 8 (0/16 samples positive); however, EHEC persisted in higher numbers (
P
= 0.05) in Myc+ shoots (4/16 positive) at days 15 and 22.
Salmonella
, likewise, survived in statistically higher numbers of Myc+ shoot samples (8/8) at day 8, compared with survival in Myc− shoots (i.e., only 4/8). These results suggest that AM fungi may potentially enhance the survival of
E. coli
O157:H7 and
Salmonella
in the stems of growing leek plants.
Metrics
Details
- Title
- Salmonella and Escherichia coli O157:H7 Survival in Soil and Translocation into Leeks (Allium porrum) as Influenced by an Arbuscular Mycorrhizal Fungus (Glomus intraradices)
- Creators
- Joshua B. Gurtler - Food Safety and Intervention Technologies Research Unit, U.S. Department of Agriculture, Agricultural Research Service (ARS), Eastern Regional Research Center, Wyndmoor, Pennsylvania, USA.David D. Douds - Eastern Regional Research CenterBrian P. Dirks - Drexel UniversityJennifer J. Quinlan - Drexel UniversityApril M. Nicholson - Delaware State UniversityJohn G. Phillips - fU.S. Department of Agriculture, ARS, Wyndmoor, Pennsylvania, USABrendan A. Niemira - Eastern Regional Research Center
- Publication Details
- Applied and environmental microbiology, v 79(6), pp 1813-1820
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Nutrition Sciences
- Web of Science ID
- WOS:000315454500006
- Scopus ID
- 2-s2.0-84874817312
- Other Identifier
- 991019169642404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology