Journal article
Sap1 Promotes the Association of the Replication Fork Protection Complex With Chromatin and Is Involved in the Replication Checkpoint in Schizosaccharomyces pombe
Genetics (Austin), v 175(2), pp 553-566
Feb 2007
PMID: 17151242
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Sap1 is involved in replication fork pausing at rDNA repeats and functions during mating-type switching in
Schizosaccharomyces pombe
. These two roles are dependent on the ability of Sap1 to bind specific DNA sequences at the rDNA and mating-type loci, respectively. In
S. pombe
, Swi1 and Swi3 form the replication fork protection complex (FPC) and play important roles in the activation of the replication checkpoint and the stabilization of stalled replication forks. Here we describe the roles of Sap1 in the replication checkpoint. We show that Sap1 is involved in the activation of the replication checkpoint kinase Cds1 and that
sap1
mutant cells accumulate spontaneous DNA damage during the S- and G2-phases, which is indicative of fork damage. We also show that
sap1
mutants have a defect in the resumption of DNA replication after fork arrest. Sap1 is localized at the replication origin
ori2004
and this localization is required for the association of the FPC with chromatin. We propose that Sap1 is required to recruit the FPC to chromatin, thereby contributing to the activation of the replication checkpoint and the stabilization of replication forks.
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Details
- Title
- Sap1 Promotes the Association of the Replication Fork Protection Complex With Chromatin and Is Involved in the Replication Checkpoint in Schizosaccharomyces pombe
- Creators
- Chiaki Noguchi - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102Eishi Noguchi - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102
- Publication Details
- Genetics (Austin), v 175(2), pp 553-566
- Publisher
- Copyright © 2007 by the Genetics Society of America
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000244689600012
- Scopus ID
- 2-s2.0-34247876071
- Other Identifier
- 991014877772804721
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- Web of Science research areas
- Genetics & Heredity