Journal article
Scalable Formation of Highly Viable and Functional Hepatocellular Carcinoma Spheroids in an Oxygen‐Permeable Microwell Device for Anti‐Tumor Drug Evaluation
Advanced healthcare materials, v 11(18), pp e2200863-n/a
21 Sep 2022
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
For high‐throughput anti‐cancer drug screening, microwell arrays may serve as an effective tool to generate uniform and scalable tumor spheroids. However, microwell arrays are commonly anchored in non‐oxygen‐permeable culture plates, leading to limited oxygen supply for avascular spheroids. Herein, a polydimethylsiloxane (PDMS)‐based oxygen‐permeable microwell device is introduced for generating highly viable and functional hepatocellular carcinoma (HCC) spheroids. The PDMS sheets at the bottom of the microwell device provide a high flux of oxygen like in vivo neighboring hepatic sinusoids. Owing to the better oxygen supply, the generated HepG2 spheroids are larger in size and exhibit higher viability and proliferation with less cell apoptosis and necrosis. These spheroids also exhibit lower levels of anaerobic cellular respiration and express higher levels of liver‐related functions. In anti‐cancer drug testing, spheroids cultured in PDMS plates show a significantly stronger resistance against doxorubicin because of the stronger stem‐cell and multidrug resistance phenotype. Moreover, higher expression of vascular endothelial growth factor‐A produces a stronger angiogenesis capability of the spheroids. Overall, compared to the spheroids cultured in conventional non‐oxygen‐permeable plates, these spheroids can be used as a more favorable model for early‐stage HCCs and be applied in high‐throughput anti‐cancer drug screening.
A polydimethylsiloxane (PDMS)‐based oxygen‐permeable microwell device is introduced for generating highly viable and functional hepatocellular carcinoma (HCC) spheroids. The PDMS sheets of the microwell device provide high flux oxygen and function like in vivo neighboring hepatic sinusoids in terms of oxygen supply. The generated spheroids can be used as a favorable model mimicking early‐stage avascular HCCs for high‐throughput drug screening.
Metrics
Details
- Title
- Scalable Formation of Highly Viable and Functional Hepatocellular Carcinoma Spheroids in an Oxygen‐Permeable Microwell Device for Anti‐Tumor Drug Evaluation
- Creators
- Jianyu He - Tsinghua UniversityChang Zhou - Tsinghua UniversityXiaolei Xu - Tsinghua UniversityZhenzhen Zhou - Ministry of EducationMathieu Danoy - University of TokyoMarie Shinohara - University of TokyoWenjin Xiao - Centre de Recherche des CordeliersDong Zhu - Tsinghua UniversityXiuying Zhao - Tsinghua UniversityXiaobin Feng - Tsinghua UniversityYilei Mao - Peking Union Medical College HospitalWei Sun - Tsinghua UniversityYasuyuki Sakai - University of TokyoHuayu Yang - Peking Union Medical College HospitalYuan Pang - Tsinghua University
- Publication Details
- Advanced healthcare materials, v 11(18), pp e2200863-n/a
- Publisher
- Wiley
- Number of pages
- 15
- Grant note
- 111 Project (B17026) Tsinghua University Peking Union Medical College Hospital Initiative Scientific Research Program (20191080843) Tsinghua University Initiative Scientific Research Program (20213080030) National Natural Science Foundation of China (52175273; 82072837; 52211540006) National Key Research and Development Program of China (2018YFA0703004) Tsinghua University Spring Breeze Fund (20201080760)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000840659800001
- Scopus ID
- 2-s2.0-85135778044
- Other Identifier
- 991019167443604721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials
- Nanoscience & Nanotechnology