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Journal article
Scavenger Receptor BI Promotes High Density Lipoprotein-mediated Cellular Cholesterol Efflux
The Journal of biological chemistry, v 272(34), pp 20982-20985
22 Aug 1997
PMID: 9261096
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Scavenger receptor BI (SR-BI) binds high density lipoproteins (HDL) with high affinity and mediates the selective uptake of HDL cholesteryl ester. We examined the potential role of SR-BI in mediating cellular cholesterol efflux. In Chinese hamster ovary cells stably transfected with murine SR-BI, overexpression of SR-BI resulted in a 3–4-fold stimulation of initial cholesterol efflux rates. Efflux rates correlated with SR-BI expression in cells and HDL concentration in the medium. When incubated with synthetic cholesterol-free HDL, SR-BI-transfected cells showed ∼3-fold increases in initial rates of efflux compared with control cells, indicating that SR-BI expression enhances net cholesterol efflux mediated by discoidal HDL. In six different cell types, including cultured macrophages, the rate of efflux of cholesterol mediated by HDL or serum was well correlated with cellular SR-BI expression level. In addition, in situhybridization experiments revealed that SR-BI mRNA was expressed in the thickened intima of atheromatous aorta of apolipoprotein E knockout mice. Thus, SR-BI is an authentic HDL receptor mediating cellular cholesterol efflux. SR-BI may facilitate the initial steps of HDL-mediated cholesterol efflux in the arterial wall as well as later steps of reverse cholesterol transport involving uptake of HDL cholesterol in the liver.
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Details
- Title
- Scavenger Receptor BI Promotes High Density Lipoprotein-mediated Cellular Cholesterol Efflux
- Creators
- Yong Ji - Columbia UniversityBo Jian - Allegheny University of the Health SciencesNan Wang - Columbia UniversityYu Sun - Columbia UniversityMargarita de la Llera Moya - Department of Biochemistry, MCP Hahnemann School of Medicine, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19129Michael C. Phillips - Allegheny University of the Health SciencesGeorge H. Rothblat - Allegheny University of the Health SciencesJohn B. Swaney - Allegheny University of the Health SciencesAlan R. Tall - Columbia UniversityYing Sun - College of Engineering (1970-)
- Publication Details
- The Journal of biological chemistry, v 272(34), pp 20982-20985
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:A1997XR78900006
- Scopus ID
- 2-s2.0-0030868610
- Other Identifier
- 991019167470904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology