Journal article
Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network
The Journal of urology, v 194(1), pp 127-135
Jul 2015
PMID: 25596358
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network to investigate a possible microbial etiology.
Male patients with urologic chronic pelvic pain syndrome and matched controls were asked to provide initial, midstream and post-prostatic massage urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between urologic chronic pelvic pain syndrome and control study participants for the presence of species or species variation in a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression.
Initial and midstream urine specimens were obtained from 110 (post-prostatic massage urine in 67) participants with urologic chronic pelvic pain syndrome and 115 (post-prostatic massage urine in 62) controls. Overall 78, 73 and 54 species (42, 39 and 27 genera) were detected in initial, midstream and post-prostatic massage urine specimens, respectively. Mean (SD) initial, midstream and post-prostatic massage urine species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33 (1.24) for cases, and 1.75 (1.32), 1.23 (1.15) and 1.56 (0.97) for controls, respectively. Overall species and genus composition differed significantly between participants with urologic chronic pelvic pain syndrome and controls in initial stream urine (p=0.002 species level, p=0.004 genus level), with Burkholderia cenocepacia overrepresented in urologic chronic pelvic pain syndrome. No significant differences were observed at any level in midstream or post-prostatic massage urine samples.
Assessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified overrepresentation of B. cenocepacia in urologic chronic pelvic pain syndrome. Future studies are planned to further evaluate microbiota associations with variable and changing urologic chronic pelvic pain syndrome symptom patterns.
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Details
- Title
- Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network
- Creators
- J Curtis Nickel - Department of Urology, Queen's University, Kingston, Ontario, Canada. Electronic address: jcn@queensu.caAlisa Stephens - Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PennsylvaniaJ Richard Landis - Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PennsylvaniaJun Chen - Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MinnesotaChris Mullins - National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MarylandAdrie van Bokhoven - Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, ColoradoM Scott Lucia - Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, ColoradoRachael Melton-Kreft - Allegheny Health Network, Center of Excellence in Biofilm Research, Pittsburgh, PennsylvaniaGarth D Ehrlich - Departments of Microbiology & Immunology and Otolaryngology-Head and Neck Surgery, Drexel University College of Medicine, Philadelphia, Pennsylvania
- Publication Details
- The Journal of urology, v 194(1), pp 127-135
- Publisher
- Elsevier; United States
- Grant note
- U01DK82345 / NIDDK NIH HHS U01 DK082342 / NIDDK NIH HHS U01DK82325 / NIDDK NIH HHS U01 DK082315 / NIDDK NIH HHS U01DK82344 / NIDDK NIH HHS U01DK82342 / NIDDK NIH HHS Z99 DK999999 / Intramural NIH HHS U01DK82316 / NIDDK NIH HHS U01DK82333 / NIDDK NIH HHS U01DK82370 / NIDDK NIH HHS UL1 TR001082 / NCATS NIH HHS U01DK82315 / NIDDK NIH HHS U01 DK103271 / NIDDK NIH HHS U01 DK082333 / NIDDK NIH HHS U01 DK082370 / NIDDK NIH HHS U01 DK082316 / NIDDK NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000356012100035
- Scopus ID
- 2-s2.0-84953361711
- Other Identifier
- 991014970023204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Urology & Nephrology