Journal article
Selective Depletion of Liver and Splenic Macrophages Using Liposomes Encapsulating the Drug Dichloromethylene Diphosphonate: Effects on Antimicrobial Resistance
Journal of leukocyte biology, v 49(6), pp 579-586
Jun 1991
PMID: 1827490
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The current results provide direct evidence for a role of tissue macrophages (Mφ) in natural immunity and support the use of immunomodulators to enhance antiviral resistance in immunocompromised individuals. In this study, macrophages (Mφ) in the spleen and liver were eliminated by intravenous (i.v.) injection of the drug dichloromethylene diphosphonate (DMDP) encapsulated in liposomes. The effect of this depletion system on peritoneal Mφ, peripheral blood leukocytes, splenic natural killer (NK) activity, and natural and immunomodulator‐induced host resistance was then assessed. Barrier‐maintained CD‐1 female mice were inoculated i.v. either with DMDP liposomes, free liposomes (containing no DMDP), or saline on day ‐2 or on days ‐3 and ‐1 before cell population analysis or infection. Single or double treatment with DMDP liposomes had no effect on peritoneal Mφ as indicated by no changes in total number, differential counts, or ectoenzyme patterns. Double treatment with DMDP liposomes caused a marked leukocytosis in blood, primarily of lymphocytes and polymorphonuclear leukocytes (PMN), and a transient depression of spontaneous and interferon‐inducible splenic NK activity. The effects on host resistance to i.v. infection with Listeria monocytogenes or herpes simplex virus type 2 (HSV‐2) indicated that i.v. treatment with DMDP liposomes significantly reduced natural resistance to these microorganisms as evidenced by increased mortality and decreased median survival time. When DMDP liposomes‐treated mice were given the immunomodulator maleic anhydride divinyl ether copolymer (MVE‐2) intraperitoneally the day before infection with HSV‐2, the immunosuppressive effect of DMDP liposome treatment was significantly reversed.
Metrics
Details
- Title
- Selective Depletion of Liver and Splenic Macrophages Using Liposomes Encapsulating the Drug Dichloromethylene Diphosphonate: Effects on Antimicrobial Resistance
- Creators
- Angelo J. Pinto - Drexel UniversityDeneen Stewart - Drexel UniversityNico RooijenPage S. Morahan - Drexel University
- Publication Details
- Journal of leukocyte biology, v 49(6), pp 579-586
- Publisher
- Wiley
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1991FL85300006
- Scopus ID
- 2-s2.0-0025780421
- Other Identifier
- 991019184021304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology
- Hematology
- Immunology