Journal article
Selective Modulation of Endothelial Cell [Ca2+]i Response to Flow by the Onset Rate of Shear Stress
Journal of biomechanical engineering, v 122(3), pp 274-282
01 Jun 2000
PMID: 10923296
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The response of endothelial cells (ECs) to their hemodynamic environment strongly influences normal vascular physiology and the pathogenesis of atherosclerosis. Unique responses to the complex flow patterns in lesion-prone regions imply that the temporal and spatial features of the mechanical stimuli modulate the cellular response to flow. We report the first systematic study of the effects of temporal gradients of shear stress on ECs. Flow was applied to cultured ECs using a novel cone-and-plate device allowing precise and independent control of the shear stress magnitude and the onset rate. Intracellular free calcium concentration [Ca2+]i increased rapidly following the onset of flow, and the characteristics of the transient were modulated by both the shear stress magnitude and onset rate. ECs were most sensitive to shear stress applied at physiological onset rates. Furthermore, the relative contribution of extracellular calcium and IP3-mediated release were dependent upon the specific flow regime. [S0148-0731(00)01003-7]
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Details
- Title
- Selective Modulation of Endothelial Cell [Ca2+]i Response to Flow by the Onset Rate of Shear Stress
- Creators
- Brett R Blackman - Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104Lawrence E Thibault - School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA 19104Kenneth A Barbee - School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA 19104
- Publication Details
- Journal of biomechanical engineering, v 122(3), pp 274-282
- Publisher
- ASME
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000167111000011
- Scopus ID
- 2-s2.0-0034210106
- Other Identifier
- 991014877653004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Engineering, Biomedical