Journal article
Selective inhibition of restriction endonuclease cleavage by DNA intercalators
Biochemical and biophysical research communications, v 115(2), pp 484-491
1983
PMID: 6312980
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The preferred dye binding sites and the microenvironment of known nucleotide sequences within mitochondrial and plasmid pBR322 DNA was probed in a gross fashion with restriction endonucleases. The intercalating dyes, ethidium bromide and propidium iodide, do not inhibit a given restriction endonuclease equally at all of the restriction sites within a DNA molecule. The selective inhibition may be explained, in part, by the potential B to Z conformation transition of DNA flanking the restriction site and by preferred dye binding sites. Propidium iodide was found to be a more potent inhibitor than ethidium bromide and the inhibition is independent of the type of cut made by the enzyme.
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Details
- Title
- Selective inhibition of restriction endonuclease cleavage by DNA intercalators
- Creators
- Gerald Soslau - Hahnemann University HospitalKathleen Pirollo - The Wistar Institute
- Publication Details
- Biochemical and biophysical research communications, v 115(2), pp 484-491
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1983RJ19700012
- Scopus ID
- 2-s2.0-0020590731
- Other Identifier
- 991019184306804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics