Journal article
Selective regulation of cytoskeletal tension and cell-matrix adhesion by RhoA and Src
Integrative biology (Cambridge), v 6(8), pp 743-754
01 Aug 2014
PMID: 24984203
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The crosstalk between cells and their microenvironment enables cellular adaptation to external mechanical cues through the remodeling of cytoskeletal structures and cell-matrix adhesions to ensure normal cell function. This study investigates the relationship between the cytosketetal tension and integrin alpha(5)beta(1) adhesion strength to the matrix (i.e. fibronectin) in the context of RhoA-Src crosstalk. Integration of atomic force microscopy (AFM) with total internal reflection fluorescence and spinning-disk confocal microscopy enabled acquisition of complementary structural and functional measurements on live vascular smooth muscle cells expressing RhoA and c-Src variants (wild-type, dominant negative, constitutively active). Single ligand-receptor interaction measurements performed with AFM probes functionalized with fibronectin showed that RhoA and c-Src activation have different effects on cytoskeletat tension development, inducing two distinct force stiffness functional regimes for alpha(5)beta(1)-integrin binding to fibronectin. Moreover, fluorescence measurements showed that c-Src activation had a modest effect on actin morphology, while RhoA significantly modulated stress fiber formation. In addition, c-Src was associated with regulation of myosin light chain (MLC) phosphorylation, suggesting a c-Src-dependent modulation of RhoA pathway through activation of downstream effectors. Therefore, c-Src may be a possible component of cytoskeletal tension regulation through MLC activation. Our findings suggest that Src and RhoA coordinate a regulatory network that determines cytoskeletal tension through activation of actomyosin contractility. In turn, the cytoskeletal tension state modulates integrin alpha(5)beta(1)-fibronectin adhesion force.
Metrics
Details
- Title
- Selective regulation of cytoskeletal tension and cell-matrix adhesion by RhoA and Src
- Creators
- Harini Sreenivasappa - Texas A&M UniversitySankar P. Chaki - Texas A&M UniversitySoon-Mi Lim - Texas A&M Health Science CenterJerome P. Trzeciakowski - Texas A&M Health Science CenterMichael W. Davidson - National High Magnetic Field LaboratoryGonzalo M. Rivera - Texas A&M UniversityAndreea Trache - Texas A&M University
- Publication Details
- Integrative biology (Cambridge), v 6(8), pp 743-754
- Publisher
- Royal Soc Chemistry
- Number of pages
- 12
- Grant note
- 0747334 / NSF CAREER; National Science Foundation (NSF); NSF - Office of the Director (OD) 0835205N / AHA-National SDG
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Cell Imaging Center
- Web of Science ID
- WOS:000339930500002
- Scopus ID
- 2-s2.0-84904723706
- Other Identifier
- 991022008089204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology