Journal article
Self-Assembly of DNA–Minocycline Complexes by Metal Ions with Controlled Drug Release
ACS applied materials & interfaces, Vol.11(33), pp.29512-29521
21 Aug 2019
PMID: 31397552
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Here we reported a study of metal ions-assisted assembly of DNA–minocycline (MC) complexes and their potential application for controlling MC release. In the presence of divalent cations of magnesium or calcium ions (M2+), MC, a zwitterionic tetracycline analogue, was found to bind to phosphate groups of nucleic acids via an electrostatic bridge of phosphate (DNA)-M2+-MC. We investigated multiple parameters for affecting the formation of DNA-Mg2+-MC complex, including metal ion concentrations, base composition, DNA length, and single- versus double-stranded DNA. For different nitrogen bases, single-stranded poly(A)20 and poly(T)20 showed a higher MC entrapment efficiency of DNA-Mg2+-MC complex than poly(C)20 and poly(G)20. Single-stranded DNA was also found to form a more stable DNA-Mg2+-MC complex than double-stranded DNA. Between different divalent metal ions, we observed that the formation of DNA-Ca2+-MC complex was more stable and efficient than the formation of DNA-Mg2+-MC complex. Toward drug release, we used agarose gel to encapsulate DNA-Mg2+-MC complexes and monitored MC release. Some DNA-Mg2+-MC complexes could prolong MC release from agarose gel to more than 10 days as compared with the quick release of free MC from agarose gel in less than 1 day. The released MC from DNA-Mg2+-MC complexes retained the anti-inflammatory bioactivity to inhibit nitric oxide production from pro-inflammatory macrophages. The reported study of metal ion-assisted DNA-MC assembly not only increased our understanding of biochemical interactions between tetracycline molecules and nucleic acids but also contributed to the development of a highly tunable drug delivery system to mediate MC release for clinical applications.
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Details
- Title
- Self-Assembly of DNA–Minocycline Complexes by Metal Ions with Controlled Drug Release
- Creators
- Ting Zhang - Rutgers, The State University of New JerseyJia Nong - Drexel UniversityNouf Alzahrani - Rutgers, The State University of New JerseyZhicheng Wang - Drexel UniversitySung Won Oh - Rutgers, The State University of New JerseyTristan Meier - Eastern Regional High School , 1401 Laurel Oak Road , Voorhees , New Jersey 08043 , United States.Dong Gyu Yang - Rutgers, The State University of New JerseyYonggang Ke - The Wallace H. Coulter Department of Biomedical EngineeringYinghui Zhong - Drexel UniversityJinglin Fu - Rutgers, The State University of New Jersey
- Publication Details
- ACS applied materials & interfaces, Vol.11(33), pp.29512-29521
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Identifiers
- 991019167556404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology