Journal article
Self-Assembly of Fiber-Forming Collagen Mimetic Peptides Controlled by Triple-Helical Nucleation
Journal of the American Chemical Society, Vol.136(41), pp.14417-14424
15 Oct 2014
PMID: 25494829
Abstract
Mimicking the multistep self-assembly of the fibrillar protein collagen is an important design challenge in biomimetic supramolecular chemistry. Utilizing the complementarity of oppositely charged domains in short collagen-like peptides, we have devised a strategy for the self-assembly of these peptides into fibers. The strategy depends on the formation of a staggered triple helical species facilitated by interchain charged pairs, and is inspired by similar sticky-ended fibrillation designs applied in DNA and coiled coil fibers. We compare two classes of collagen mimetic peptides with the same composition but different domain arrangements, and show that differences in their proposed nucleation events differentiates their fibrillation capabilities. Larger nucleation domains result in rapid fiber formation and eventual precipitation or gelation while short nucleation domains leave the peptide soluble for long periods of time. For one of the fiber-forming peptides, we elucidate the packing parameters by X-ray diffraction.
Metrics
1 Record Views
Details
- Title
- Self-Assembly of Fiber-Forming Collagen Mimetic Peptides Controlled by Triple-Helical Nucleation
- Creators
- Biplab Sarkar - Rice UniversityLesley E. R. O'Leary - Rice UniversityJeffrey D. Hartgerink - Rice University
- Publication Details
- Journal of the American Chemical Society, Vol.136(41), pp.14417-14424
- Publisher
- Amer Chemical Soc
- Number of pages
- 8
- Grant note
- DMR-1206899 / National Science Foundation; National Science Foundation (NSF) C1557 / Robert A. Welch Foundation; The Welch Foundation 1206899 / Division Of Materials Research; National Science Foundation (NSF); NSF - Directorate for Mathematical & Physical Sciences (MPS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Identifiers
- 991021956615404721
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Web of Science research areas
- Chemistry, Multidisciplinary