Journal article
Self-Assembly of a Dentinogenic Peptide Hydrogel
ACS omega, Vol.3(6), pp.5980-5987
30 Jun 2018
PMID: 30023936
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Current standard of care for treating infected dental pulp, root canal therapy, retains the physical properties of the tooth to a large extent, but does not aim to rejuvenate the pulp tissue. Tissue-engineered acellular biomimetic hydrogels have great potential to facilitate the regeneration of the tissue through the recruitment of autologous stem cells. We propose the use of a dentinogenic peptide that self-assembles into ss-sheet-based nanofibers that constitute a biodegradable and injectable hydrogel for support of dental pulp stem cells. The peptide backbone contains a ss-sheet-forming segment and a matrix extracellular phosphoglycoprotein mimic sequence at the C-terminus. The high epitope presentation of the functional moiety in the self-assembled nanofibers may enable recapitulation of a functional niche for the survival and proliferation of autologous cells. We elucidated the hierarchical self-assembly of the peptide through biophysical techniques, including scanning electron microscopy and atomic force microscopy. The material property of the self-assembled hydrogel was probed though oscillatory rheometry, demonstrating its thixotropic nature. We also demonstrate the cytocompatibility of the hydrogel with respect to fibroblasts and dental pulp stem cells. The self-assembled peptide platform holds promise for guided dentinogenesis and it can be tailored to a variety of applications in soft tissue engineering and translational medicine in the future.
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Details
- Title
- Self-Assembly of a Dentinogenic Peptide Hydrogel
- Creators
- Peter K. Nguyen - New Jersey Institute of TechnologyWilliam Gao - New Jersey Institute of TechnologySaloni D. Patel - New Jersey Institute of TechnologyZain Siddiqui - New Jersey Institute of TechnologySaul Weiner - Rutgers, The State University of New JerseyEmi Shimizu - Rutgers, The State University of New JerseyBiplab Sarkar - New Jersey Institute of TechnologyVivek A. Kumar - Rutgers, The State University of New Jersey
- Publication Details
- ACS omega, Vol.3(6), pp.5980-5987
- Publisher
- Amer Chemical Soc
- Number of pages
- 8
- Grant note
- R01DE025885 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) NJIT
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Identifiers
- 991021956861404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary