Logo image
Back
Senescence associated secretory phenotype profile from primary lung mice fibroblasts depends on the senescence induction stimuli
Journal article   Open access

Senescence associated secretory phenotype profile from primary lung mice fibroblasts depends on the senescence induction stimuli

L. A. Maciel-Baron, S. L. Morales-Rosales, A. A. Aquino-Cruz, F. Triana-Martinez, S. Galvan-Arzate, A. Luna-Lopez, V. Y. Gonzalez-Puertos, N. E. Lopez-Diazguerrero, C. Torres and Mina Koenigsberg
AGE, v 38(1)
01 Feb 2016
DOI: https://doi.org/10.1007/s11357-016-9886-1
PMID: 26867806
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1007/s11357-016-9886-1View
Published, Version of Record (VoR)Open Access (License Unspecified),  Open

Abstract

Geriatrics & Gerontology Life Sciences & Biomedicine Science & Technology
Cellular senescence is a multifactorial phenomenon of growth arrest and distorted function, which has been recognized as an important feature during tumor suppression mechanisms and a contributor to aging. Senescent cells have an altered secretion pattern called Senescence-Associated Secretory Phenotype (SASP) that comprises a complex mix of factors including cytokines, growth factors, chemokines, and matrix metalloproteinases. SASP has been related with local inflammation that leads to cellular transformation and neurodegenerative diseases. Various pathways for senescence induction have been proposed; the most studied is replicative senescence due to telomere attrition called replicative senescence (RS). However, senescence can be prematurely achieved when cells are exposed to diverse stimuli such as oxidative stress (stressinduced premature senescence, SIPS) or proteasome inhibition (proteasome inhibition-induced premature senescence, PIIPS). SASP has been characterized in RS and SIPS but not in PIIPS. Hence, our aim was to determine SASP components in primary lung fibroblasts obtained from CD-1 mice induced to senescence by PIIPS and compare them to RS and SIPS. Our results showed important variations in the 62 cytokines analyzed, while SIPS and RS showed an increase in the secretion of most cytokines, and in PIIPS only 13 were incremented. Variations in glutathione-redox balance were also observed in SIPS and RS, and not in PIIPS. All senescence types SASP displayed a pro-inflammatory profile and increased proliferation in L929 mice fibroblasts exposed to SASP. However, the behavior observed was not exactly the same, suggesting that the senescence induction pathway might encompass dissimilar responses in adjacent cells and promote different outcomes.

Metrics

9 Record Views
82 citations in Web of Science
82 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Geriatrics & Gerontology
Logo image