Journal article
Separation of very hydrophobic compounds by hydrophobic interaction electrokinetic chromatography
Journal of Chromatography A, v 680(1), pp 73-83
30 Sep 1994
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Separations of very hydrophobic neutral analytes were achieved using hydrophobic interaction electrokinetic chromatography (HI-EKC). Alkyl aryl ketone homologues dodecanophenone (C
18), tetradecanophenone (C
20), hexadecanophenone (C
22) and octadecanophenone (C
24) were separated via hydrophobic interactions between free sodium dodecyl sulfate (SDS) monomers and the analytes. The first running buffer consisted of 50 m
M SDS and 50% acetonitrile (pH 7.0). A complete reversal in the elution order of these analytes was obtained with the second running buffer, 20 m
M cetyltrimethylammonium bromide (CTAB) and 50% acetonitrile (pH 2.8). With the second running buffer, electroosmotic flow was suppressed and the free CTAB monomers migrated toward the detector. Through hydrophobic interactions between the free CTAB monomers and the analytes, separations of these very hydrophobic alkyl aryl ketones were obtained in less than 10 min; analysis times were less than 5 min with the SDS-based separations.
Metrics
Details
- Title
- Separation of very hydrophobic compounds by hydrophobic interaction electrokinetic chromatography
- Creators
- Eric S. Ahuja - Villanova UniversityJoe P. Foley - Villanova University
- Publication Details
- Journal of Chromatography A, v 680(1), pp 73-83
- Publisher
- Elsevier
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:A1994PL67300010
- Scopus ID
- 2-s2.0-0028030548
- Other Identifier
- 991021906105004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical