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Journal article
Separation of weak acids, neutral compounds, and permanent anions using sequential elution liquid chromatography with tandem columns
Journal of chromatography A, v 1731, p465178
25 Jul 2024
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Abstract
This paper discusses the development of an analytical method by an alternative separation approach, sequential elution liquid chromatography (SE-LC), to separate permanently charged ions (anions), weak acids, and neutral compounds using anion exchange and reversed-phase columns in tandem. SE-LC separates classes of compounds by group by employing two or more elution modes. Advantages to using SE-LC over conventional HPLC are a greater peak capacity and a reduced separation disorder. Importantly, the same HPLC as used for a conventional HPLC separation may be used to afford a successful SE-LC separation. Mobile phase selection and gradient optimization are integral for a successful SE-LC class separation of permanent anions, weak acids, and neutral compounds and will be discussed in detail in this paper. The most successful (best resolution and repeatability) SE-LC separation was achieved by applying isocratic elution at low pH to elute the weak acids, followed by an acetonitrile gradient to elute the neutral compounds, and last a sodium methanesulfonate gradient to elute the anionic compounds using a superficially porous C18 column coupled with a strong anion exchange (SAX) column. Repeatability (RSD) in the retention times and peak areas of the analytes was less than 0.25% and 1.5%, respectively.
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Details
- Title
- Separation of weak acids, neutral compounds, and permanent anions using sequential elution liquid chromatography with tandem columns
- Creators
- Joe Preston Foley (Corresponding Author) - Drexel University, ChemistryLauren Kline Lovejoy - Drexel University, Chemistry
- Publication Details
- Journal of chromatography A, v 1731, p465178
- Publisher
- Elsevier
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:001281184100001
- Scopus ID
- 2-s2.0-85199427398
- Other Identifier
- 991021893408504721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical