Journal article
Septin-Driven Coordination of Actin and Microtubule Remodeling Regulates the Collateral Branching of Axons
Current biology, v 22(12), pp 1109-1115
19 Jun 2012
PMID: 22608511
Abstract
Axon branching is fundamental to the development of the peripheral and central nervous system [1, 2]. Branches that sprout from the axon shaft are termed collateral or interstitial branches [3, 4]. Collateral branching of axons requires the formation of filopodia from actin microfilaments (F-actin) and their engorgement with microtubules (MTs) that splay from the axon shaft [4–6]. The mechanisms that drive and coordinate the remodeling of actin and MTs during branch morphogenesis are poorly understood. Septins comprise a family of GTP-binding proteins that oligomerize into higher-order structures, which associate with membranes and the actin and microtubule cytoskeleton [7, 8]. Here, we show that collateral branching of axons requires SEPT6 and SEPT7, two interacting septins [9]. In the axons of sensory neurons, both SEPT6 and SEPT7 accumulate at incipient sites of filopodia formation. We show that SEPT6 localizes to axonal patches of F-actin and increases the recruitment of cortactin, a regulator of Arp2/3-mediated actin polymerization, triggering the emergence of filopodia. Conversely, SEPT7 promotes the entry of axonal MTs into filopodia, enabling the formation of collateral branches. Surprisingly, septins provide a novel mechanism for the collateral branching of axons by coordinating the remodeling of the actin and microtubule cytoskeleton.
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► Collateral branching of axons is regulated by SEPT6 and SEPT7 ► SEPT6 and SEPT7 are recruited to nascent sites of filopodia formation ► SEPT6 recruits cortactin to patches of F-actin and triggers filopodia formation ► SEPT7 alters axonal MT organization, triggering MT entry into filopodia
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Details
- Title
- Septin-Driven Coordination of Actin and Microtubule Remodeling Regulates the Collateral Branching of Axons
- Creators
- Jianli Hu - Department of Biology, Drexel University, Philadelphia, PA 19104, USAXiaobo Bai - Department of Biology, Drexel University, Philadelphia, PA 19104, USAJonathan R Bowen - Department of Biology, Drexel University, Philadelphia, PA 19104, USALee Dolat - Department of Biology, Drexel University, Philadelphia, PA 19104, USAFarida Korobova - Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USAWenqian Yu - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USAPeter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USATatyana Svitkina - Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USAGianluca Gallo - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USAElias T Spiliotis - Department of Biology, Drexel University, Philadelphia, PA 19104, USA
- Publication Details
- Current biology, v 22(12), pp 1109-1115
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology; Neurobiology and Anatomy
- Web of Science ID
- WOS:000305766900026
- Scopus ID
- 2-s2.0-84862645233
- Other Identifier
- 991014878028304721
InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology
- Cell Biology