Journal article
Septin GTPases spatially guide microtubule organization and plus end dynamics in polarizing epithelia
The Journal of cell biology, Vol.194(2), pp.187-197
25 Jul 2011
PMCID: PMC3144415
PMID: 21788367
Abstract
Establishment of epithelial polarity requires the reorganization of the microtubule (MT) cytoskeleton from a radial array into a network positioned along the apicobasal axis of the cell. Little is known about the mechanisms that spatially guide the remodeling of MTs during epithelial polarization. Septins are filamentous guanine triphosphatases (GTPases) that associate with MTs, but the function of septins in MT organization and dynamics is poorly understood. In this paper, we show that in polarizing epithelia, septins guide the directionality of MT plus end movement by suppressing MT catastrophe. By enabling persistent MT growth, two spatially distinct populations of septins, perinuclear and peripheral filaments, steer the growth and capture of MT plus ends. This navigation mechanism is essential for the maintenance of perinuclear MT bundles and for the orientation of peripheral MTs as well as for the apicobasal positioning of MTs. Our results suggest that septins provide the directional guidance cues necessary for polarizing the epithelial MT network.
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Details
- Title
- Septin GTPases spatially guide microtubule organization and plus end dynamics in polarizing epithelia
- Creators
- Jonathan R Bowen - Department of Biology, Drexel University, Philadelphia, PA 19104Daniel Hwang - Department of Biology, Drexel University, Philadelphia, PA 19104Xiaobo Bai - Department of Biology, Drexel University, Philadelphia, PA 19104Dheeraj Roy - Department of Biology, Drexel University, Philadelphia, PA 19104Elias T Spiliotis - Department of Biology, Drexel University, Philadelphia, PA 19104
- Publication Details
- The Journal of cell biology, Vol.194(2), pp.187-197
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Identifiers
- 991014877827104721
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