Journal article
Septins Recognize and Entrap Dividing Bacterial Cells for Delivery to Lysosomes
Cell host & microbe, v 24(6), pp 866-874
12 Dec 2018
PMID: 30543779
Abstract
The cytoskeleton occupies a central role in cellular immunity by promoting bacterial sensing and antibacterial functions. Septins are cytoskeletal proteins implicated in various cellular processes, including cell division. Septins also assemble into cage-like structures that entrap cytosolic Shigella, yet how septins recognize bacteria is poorly understood. Here, we discover that septins are recruited to regions of micron-scale membrane curvature upon invasion and division by a variety of bacterial species. Cardiolipin, a curvature-specific phospholipid, promotes septin recruitment to highly curved membranes of Shigella, and bacterial mutants lacking cardiolipin exhibit less septin cage entrapment. Chemically inhibiting cell separation to prolong membrane curvature or reducing Shigella cell growth respectively increases and decreases septin cage formation. Once formed, septin cages inhibit Shigella cell division upon recruitment of autophagic and lysosomal machinery. Thus, recognition of dividing bacterial cells by the septin cytoskeleton is a powerful mechanism to restrict the proliferation of intracellular bacterial pathogens.
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•Septins are recruited to micron-scale curvature of dividing intracellular bacteria•Cardiolipin promotes the recruitment of septins to curved bacterial membranes•Following recruitment, septins assemble into cages around growing bacterial cells•Septins inhibit bacterial division via recruitment of autophagic and lysosomal machinery
Septins are cytoskeleton components widely recognized for their role in eukaryotic cell division. Krokowski et al. discover that septins recognize dividing bacterial cells for entrapment and delivery to lysosomes. These results reveal a fundamental danger signal used by the host cell to recognize intracellular bacterial pathogens for cellular immunity.
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Details
- Title
- Septins Recognize and Entrap Dividing Bacterial Cells for Delivery to Lysosomes
- Creators
- Sina Krokowski - Section of Microbiology, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UKDamián Lobato-Márquez - Section of Microbiology, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UKArnaud Chastanet - MICALIS, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas 78350, FrancePedro Matos Pereira - Quantitative Imaging and NanoBiophysics Group, MRC Laboratory for Molecular Cell Biology and Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UKDimitrios Angelis - Department of Biology, Drexel University, Philadelphia, PA 19104, USADieter Galea - Section of Microbiology, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UKGerald Larrouy-Maumus - Faculty of Natural Sciences, Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UKRicardo Henriques - Quantitative Imaging and NanoBiophysics Group, MRC Laboratory for Molecular Cell Biology and Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UKElias T Spiliotis - Department of Biology, Drexel University, Philadelphia, PA 19104, USARut Carballido-López - MICALIS, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas 78350, FranceSerge Mostowy - Section of Microbiology, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UK
- Publication Details
- Cell host & microbe, v 24(6), pp 866-874
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology; Pediatrics
- Web of Science ID
- WOS:000453027400013
- Scopus ID
- 2-s2.0-85057417734
- Other Identifier
- 991014877658604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology
- Parasitology
- Virology