Journal article
Septins promote stress fiber-mediated maturation of focal adhesions and renal epithelial motility
The Journal of cell biology, v 207(2)
27 Oct 2014
PMID: 25349260
Abstract
Organogenesis and tumor metastasis involve the transformation of epithelia to highly motile mesenchymal-like cells. Septins are filamentous G proteins, which are overexpressed in metastatic carcinomas, but their functions in epithelial motility are unknown. Here, we show that a novel network of septin filaments underlies the organization of the transverse arc and radial (dorsal) stress fibers at the leading lamella of migrating renal epithelia. Surprisingly, septin depletion resulted in smaller and more transient and peripheral focal adhesions. This phenotype was accompanied by a highly disorganized lamellar actin network and rescued by the actin bundling protein α-actinin-1. We show that preassembled actin filaments are cross-linked directly by Septin 9 (SEPT9), whose expression is increased after induction of renal epithelial motility with the hepatocyte growth factor. Significantly, SEPT9 overexpression enhanced renal cell migration in 2D and 3D matrices, whereas SEPT9 knockdown decreased migration. These results suggest that septins promote epithelial motility by reinforcing the cross-linking of lamellar stress fibers and the stability of nascent focal adhesions.
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Details
- Title
- Septins promote stress fiber-mediated maturation of focal adhesions and renal epithelial motility
- Creators
- Lee Dolat - Department of Biology, Drexel University, Philadelphia, PA 19104John L Hunyara - Department of Biology, Drexel University, Philadelphia, PA 19104Jonathan R Bowen - Department of Biology, Drexel University, Philadelphia, PA 19104Eva Pauline Karasmanis - Department of Biology, Drexel University, Philadelphia, PA 19104Maha Elgawly - Department of Biology, Drexel University, Philadelphia, PA 19104Vitold E Galkin - Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA 23507Elias T Spiliotis - Department of Biology, Drexel University, Philadelphia, PA 19104 ets33@drexel.edu
- Publication Details
- The Journal of cell biology, v 207(2)
- Publisher
- United States
- Grant note
- F31 CA176910 / NCI NIH HHS R01 GM097664 / NIGMS NIH HHS CA176910 / NCI NIH HHS GM097664 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000343964900009
- Scopus ID
- 2-s2.0-84910683243
- Other Identifier
- 991014878064204721
InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology