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Sequence Analysis of the ZFY and Sox Genes in the Turtle, Chelydra serpentina
Journal article   Peer reviewed

Sequence Analysis of the ZFY and Sox Genes in the Turtle, Chelydra serpentina

Loretta D. Spotila, Norbert F. Kaufer, Edward Theriot, Kathleen M. Ryan, David Penick and James R. Spotila
Molecular phylogenetics and evolution, v 3(1), pp 1-9
Mar 1994
PMID: 8025725

Abstract

We have sequenced regions of the ZFY and Sox genes in the turtle Chelydra serpentina, a reptile with temperature-dependent sex determination. The FY gene in mammals encodes a transcription factor with multiple zinc fingers that may be involved in spermatogenesis as well as other processes. The turtle homologue, Zft, is 92% identical to the ZFY gene at the nucleotide and amino acid levels in the region of zinc fingers 7-12. There are several Sox genes in the turtle that are only 57-70% identical at the nucleotide level and about 55% identical at the amino acid level to the human sex-determining SRY gene. However, the turtle Sox genes, termed TSox, have the conserved motif called the HMG-box (for high mobility group DNA-binding protein) that defines a probable DNA-binding region, and thus are in the same gene family as the Sox genes of other organisms from Drosophila to man. One TSox sequence is identical at the amino acid level to a sequence found in birds, and is 98% identical to a sequence encoded autosomally in mouse and in man. The extent of sequence conservation among the Sox genes suggests that some of their functions may be conserved. Phylogenetic analysis of available Sox sequences including SRY (Sry) sequences suggests that there was a high degree of divergence between any possible immediate common ancestor of the turtle Sox sequences and the SRY (Sry) sequences.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Evolutionary Biology
Genetics & Heredity
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