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Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean
Journal article   Peer reviewed

Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean

Kyaw Thu Moe, Jong-Wook Chung, Young-Il Cho, Jung-Kyung Moon, Ja-Hwan Ku, Jin-Kyo Jung, Jungran Lee and Yong-Jin Park
Journal of integrative plant biology, v 53(1), pp 63-73
01 Jan 2011
PMID: 21205180

Abstract

Biochemistry & Molecular Biology Life Sciences & Biomedicine Plant Sciences Science & Technology
Mungbean (Vigna radiata (L.) Wilczek) is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs (>= 500 bp) with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat (SSR) motifs was identified in Jangan (1 630) compared with that of Sunhwa (1 334). A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms (SNPs) and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was similar to 860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Plant Sciences
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