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Sequence and expression analysis of WT1 and Sox9 in the red-eared slider turtle, Trachemys scripta
Journal article

Sequence and expression analysis of WT1 and Sox9 in the red-eared slider turtle, Trachemys scripta

L D Spotila, J R Spotila and S E Hall
The Journal of experimental zoology, v 281(5), pp 417-427
01 Aug 1998
DOI: https://doi.org/10.1002/(sici)1097-010x(19980801)281:5<417::aid-jez7>3.0.co;2-r
PMID: 9662829
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Amino Acid Sequence High Mobility Group Proteins - metabolism Sex Differentiation - genetics Molecular Sequence Data Male Transcription Factors - genetics DNA Primers - chemistry DNA-Binding Proteins - genetics Turtles - metabolism Sequence Analysis, DNA DNA-Binding Proteins - metabolism Transcription Factors - metabolism Turtles - genetics Animals In Situ Hybridization Sex Determination Processes RNA, Complementary - genetics Gene Expression Regulation, Developmental Base Sequence Cloning, Molecular Female WT1 Proteins SOX9 Transcription Factor High Mobility Group Proteins - genetics
Temperature-dependent sex-determination (TSD) is a phenomenon that has been characterized at the ecological, morphological, and endocrinological levels in some reptilian species. We have begun to investigate TSD at the level of molecular development by cloning, sequencing, and analyzing the expression of two genes, WT1 and Sox9, in the red-eared slider turtle Trachemys scripta. We obtained almost full-length cDNA clones for WT1 and Sox9 that were greater than 73% identical to the human homologues at the nucleotide level. WT1 was expressed in urogenital tissue at all developmental stages examined (Yntema stages 12-20) at incubation temperatures that produce males (26 degrees C) or females (32 degrees C). Sox9 was also expressed throughout these same stages, but some differences were observed. At both 26 degrees C and 32 degrees C Sox9 was expressed in the mesonephroi and the undifferentiated gonads until Yntema stage 20, when only the gonad from the 26 degrees C embryos expressed a high level. In addition, there were two transcripts of Sox9 at all stages, but the relative proportion of the two transcripts differed at the two temperatures. Although the similarities in gene expression between a TSD species and other species with genotypically determined sex probably reflect the common features of organogenesis, differences may illustrate unique mechanisms for TSD.

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Collaboration types
Domestic collaboration
Web of Science research areas
Zoology
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