Journal article
Serum Response Factor Protects Retinal Ganglion Cells Against High-Glucose Damage
Journal of molecular neuroscience, v 59(2), pp 232-240
Jun 2016
PMID: 26803311
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Serum response factor (SRF), which encodes the MADS-box family of related proteins, is a common transcription factor related to the expression of genes associated with cell survival. However, SRF's role in retinal ganglion cells (RGCs) after high-glucose injury remains unclear. In this study, we investigate the protective role of SRF after high-glucose injury and its underlying mechanism. The in vitro RGC model subjected to high glucose was established by employing a 50 mmol/L glucose culture environment. As detected by real-time quantitative PCR and Western blot, SRF was significantly upregulated in RGCs treated with high glucose. Overexpression of SRF significantly promoted survival among RGCs exposed to high glucose and inhibited RGC apoptosis. Knockdown of SRF exerted an inverse effect. Moreover, SRF upregulation enhanced expression of an antioxidant protein, nuclear factor erythroid 2-related factor (Nrf2), via control of the Fos-related antigen 1 (Fra-1). SRF upregulation also affected RGC survival after high-glucose treatment. Our findings showed that overexpression of SRF promoted survival of RGCs after high-glucose injury by regulating Fra-1 and Nrf2.
Metrics
Details
- Title
- Serum Response Factor Protects Retinal Ganglion Cells Against High-Glucose Damage
- Creators
- Yan Cao - Xi’an NO.1 HospitalLiang Wang - Xi’an NO.1 HospitalJunhong Zhao - Xi’an NO.1 HospitalHongbing Zhang - Xi’an NO.1 HospitalYing Tian - Xi’an NO.1 HospitalHoucheng Liang - Xi’an NO.1 HospitalQiang Ma - Xi’an NO.1 Hospital
- Publication Details
- Journal of molecular neuroscience, v 59(2), pp 232-240
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000380720600007
- Scopus ID
- 2-s2.0-84955240135
- Other Identifier
- 991019320714904721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences