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Journal article
Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers
Bioanalysis, v 7(22), pp 2895-2911
2015
PMID: 26394123
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC-MS can provide misleading results.
Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [(13)C6(15)N2]-lysine and [(13)C9(15)N1]-tyrosine in human cells. A stable isotope dilution LC-MS method for serum ApoA-1 was validated and levels analyzed for 50 nonsmokers and 50 smokers.
The concentration of ApoA-1 in nonsmokers was 169.4 mg/dl with an 18.4% reduction to 138.2 mg/dl in smokers. The validated assay will have clinical utility for assessing effects of smoking cessation and therapeutic or dietary interventions in high-risk populations.
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Details
- Title
- Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers
- Creators
- Qingqing Wang - Translational TherapeuticsSuhong Zhang - Translational TherapeuticsLili Guo - Translational TherapeuticsChristine M Busch - Translational TherapeuticsWenying Jian - Johnson & JohnsonNaidong Weng - Johnson & JohnsonNathaniel W Snyder - Drexel UniversityKannan Rangiah - Translational TherapeuticsClementina Mesaros - Translational TherapeuticsIan A Blair - Translational Therapeutics
- Publication Details
- Bioanalysis, v 7(22), pp 2895-2911
- Grant note
- P30 ES013508 / NIEHS NIH HHS P30ES013508 / NIEHS NIH HHS T32 ES019851 / NIEHS NIH HHS T32ES019851 / NIEHS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- A.J. Drexel Autism Institute
- Web of Science ID
- WOS:000365625700007
- Scopus ID
- 2-s2.0-84948133553
- Other Identifier
- 991019167903904721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical