Shikonin induces apoptosis and G0/G1 phase arrest of gallbladder cancer cells via the JNK signaling pathway

Tianyu Zhai; Zhenyu Hei; Qiang Ma; Haibin Liang; Yi Xu; Yichi Zhang; Longyang Jin; Chao Han; Jiandong Wang; Hualou Liang

doi:10.3892/or.2017.6038

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Shikonin induces apoptosis and G0/G1 phase arrest of gallbladder cancer cells via the JNK signaling pathway

Journal article

Open access

Peer reviewed

Shikonin induces apoptosis and G0/G1 phase arrest of gallbladder cancer cells via the JNK signaling pathway

Tianyu Zhai, Zhenyu Hei, Qiang Ma, Haibin Liang, Yi Xu, Yichi Zhang, Longyang Jin, Chao Han, Jiandong Wang and Hualou Liang

Oncology reports, v 38(6), pp 3473-3480

Dec 2017

DOI: https://doi.org/10.3892/or.2017.6038

PMID: 29039581

Featured in Collection : UN Sustainable Development Goals @ Drexel

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Abstract

Animals

Apoptosis - drug effects

Caspases - genetics

Cell Line, Tumor

Cell Proliferation - drug effects

G1 Phase Cell Cycle Checkpoints - drug effects

Gallbladder Neoplasms - drug therapy

Gallbladder Neoplasms - genetics

Gallbladder Neoplasms - pathology

Humans

MAP Kinase Signaling System - drug effects

Membrane Potential, Mitochondrial - drug effects

Mice

Naphthoquinones - administration & dosage

Xenograft Model Antitumor Assays

Shikonin, a natural product isolated from the roots of Lithospermum erythrorhizon, is considered to have antitumor effects. Gallbladder cancer (GBC) is a prevalent biliary tract malignancy with few curative therapeutic stragegies and poor prognosis. In the present study, we detected the effects of shikonin on GBC cells as well as the underlying molecular mechanisms. The results demonstrated that GBC cell proliferation was inhibited by shikonin as determined by MTT and colony formation assays. Flow cytometry results demonstrated that shikonin treatment enhanced apoptosis and promoted G0/G1 phase arrest in the GBC cells. Western blot assay showed that shikonin induced mitochondrial-dependent apoptosis via the JNK signaling pathway. Moreover, shikonin suppressed tumor growth in mice bearing GBC-derived xenografts in a dose‑dependent manner without side-effects. These results revealed that shikonin exhibits anticancer effects on GBC cells by inducing apoptosis and regulating the cell cycle. Taken together, shikonin may be a novel and safe chemotherapeutic agent for the treatment of GBC.

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34 citations in Scopus

Details

Title: Shikonin induces apoptosis and G0/G1 phase arrest of gallbladder cancer cells via the JNK signaling pathway
Creators: Tianyu Zhai - XinHua Hospital
Zhenyu Hei - XinHua Hospital
Qiang Ma - XinHua Hospital
Haibin Liang - XinHua Hospital
Yi Xu - XinHua Hospital
Yichi Zhang - XinHua Hospital
Longyang Jin - XinHua Hospital
Chao Han - XinHua Hospital
Jiandong Wang - XinHua Hospital
Hualou Liang - School of Biomedical Engineering, Science, and Health Systems (1997-)
Publication Details: Oncology reports, v 38(6), pp 3473-3480
Resource Type: Journal article
Language: English
Academic Unit: School of Biomedical Engineering, Science, and Health Systems
Web of Science ID: WOS:000417320500021
Scopus ID: 2-s2.0-85032640992
Other Identifier: 991019320403504721

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Web of Science research areas: Oncology