Journal article
Should we consider subcellular compartmentalization of metabolites, and if so, how do we measure them?
Current opinion in clinical nutrition and metabolic care, v 22(5), pp 347-354
01 Sep 2019
PMID: 31365463
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Purpose of review To examine the consequences of metabolism compartmentalized at the subcellular level, provide prototypical examples of compartmentalized metabolism, and describe methods to examine compartmentalized metabolism. Recent findings Progress in metabolomics and isotope tracing has underscored the importance of subcellular compartments of metabolism. The discovery of biological effects of metabolites as bioenergetic intermediates, anabolic building blocks, signaling mediators, and effectors in posttranslation modifications of proteins and nucleic acids have highlighted the role of compartmentalization in determining metabolic fate. Recent advances in both direct and indirect methods to quantify compartmentalized metabolism have improved upon historical approaches. Genetically encoded metabolite sensors, chemical probes, immunoaffinity purification, and compartment-resolved metabolic modeling have all been recently applied to study compartmentalization. Accurate measurement of metabolites in distinct subcellular compartments is important for understanding and pharmacologically targeting metabolic pathways in diverse disease contexts, including cancer, diabetes, heart failure, obesity, and regulation of the immune system. Direct and indirect approaches to quantify compartmentalized metabolism are advancing rapidly. Yet, major challenges remain in the generalizability, rigor, and interpretation of data from the available methods to quantify compartmentalized metabolism.
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Details
- Title
- Should we consider subcellular compartmentalization of metabolites, and if so, how do we measure them?
- Creators
- Kathryn E. Wellen - Abramson Family Cancer Research Institute, University of Pennsylvania Perelman School of Medicine.Nathaniel W. Snyder - Drexel University
- Publication Details
- Current opinion in clinical nutrition and metabolic care, v 22(5), pp 347-354
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 8
- Grant note
- R03HD092630; R01DK116005; R01CA228339 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01GM132261 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01CA228339 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R01DK116005 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) R03HD092630 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- A.J. Drexel Autism Institute
- Web of Science ID
- WOS:000501363300005
- Scopus ID
- 2-s2.0-85070875383
- Other Identifier
- 991019167983404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Endocrinology & Metabolism
- Nutrition & Dietetics