Journal article
Sialic acid in the lipopolysaccharide of Haemophilus influenzae: strain distribution, influence on serum resistance and structural characterization
Molecular microbiology, v 33(4), pp 679-692
Aug 1999
PMID: 10447878
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A survey of Haemophilus influenzae strains indicated that around one‐third of capsular strains and over two‐thirds of non‐typeable strains included sialic acid in their lipopolysaccharides (LPS). Mutation of the CMP‐Neu5Ac synthetase gene (siaB) resulted in a sialylation‐deficient phenotype. Isogenic pairs, wild type and siaB mutant of two non‐typeable strains were used to demonstrate that sialic acid influences resistance to the killing effect of normal human serum but has little effect on attachment to, or invasion of, cultured human epithelial cells or neutrophils. We determine for the first time the site of attachment of sialic acid in the LPS of a non‐typeable strain and report that a small proportion of glycoforms include two sialic acid residues in a disaccharide unit.
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Details
- Title
- Sialic acid in the lipopolysaccharide of Haemophilus influenzae: strain distribution, influence on serum resistance and structural characterization
- Creators
- Derek W HoodKatherine MakepeaceMary E DeadmanRichard F RestPierre ThibaultAdele MartinJames C RichardsE. Richard Moxon
- Publication Details
- Molecular microbiology, v 33(4), pp 679-692
- Publisher
- Blackwell Science Ltd; Oxford BSL
- Number of pages
- 14
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000082478000002
- Scopus ID
- 2-s2.0-0032773667
- Other Identifier
- 991014878592804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Microbiology