Journal article
Simian immunodeficiency virus envelope compartmentalizes in brain regions independent of neuropathology
Journal of neurovirology, v 12(2), pp 73-89
2006
PMID: 16798669
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Simian immunodeficiency virus (SIV) and human immunodeficiency virus (HIV) gp160s obtained from the brain are often genetically distinct from those isolated from other organs, suggesting the presence of brain-specific selective pressures or founder effects that result in the compartmentalization of viral quasispecies. Whereas HIV has also been found to compartmentalize within different regions of the brain, the extent of brain-regional compartmentalization of SIV in rhesus macaques has not been characterized. Furthermore, much is still unknown about whether phenotypic differences exist in envelopes from different brain regions. To address these questions, env DNA sequences were amplified from four SIVmac239-infected macaques and subjected to phylogenetic and phenetic analysis. The authors demonstrated that sequences from different areas of the brain form distinct clades, and that the long-term progressing macaques demonstrated a greater degree of regional compartmentalization compared to the rapidly progressing macaques. In addition, regional compartmentalization occurred regardless of the presence of giant-cell encephalitis. Nucleotide substitution rates at synonymous and nonsynonymous sites (ds:dn rates) indicated that positive selection varied among envelopes from different brain regions. In one macaque, envelopes from some but not all brain regions acquired changes in a conserved CD4-binding motif GGGDPE at amino acids 382 to 387. Furthermore, gp160s with the mutation G383E were able to mediate cell-to-cell fusion in a CD4-independent manner and were more susceptible to fusion inhibition by pooled plasma from infected macaques. Reversion of this mutation by site-directed mutagenesis resulted in reduction of CD4-independence and resistance to fusion inhibition in cell fusion assays. These studies demonstrate that SIV evolution within the brain results in a heterogeneous viral population with different phenotypes among different regions.
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Details
- Title
- Simian immunodeficiency virus envelope compartmentalizes in brain regions independent of neuropathology
- Creators
- Maria F Chen - Department of Neurology, University of Pennsylvania School of Medicine, PhiladelphiaUSASusan Westmoreland - Center for Comparative Medicine, Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USAElena V Ryzhova - Department of Neurology, University of Pennsylvania School of Medicine, PhiladelphiaUSAJulio Martín-García - Department of Neurology, University of Pennsylvania School of Medicine, PhiladelphiaUSASamantha S Soldan - Department of Neurology, University of Pennsylvania School of Medicine, PhiladelphiaUSAAndrew Lackner - Tulane National Primate Research Center, New Orleans, Louisiana, USAFrancisco González-Scarano - Department of Neurology, University of Pennsylvania School of Medicine, PhiladelphiaUSA
- Publication Details
- Journal of neurovirology, v 12(2), pp 73-89
- Publisher
- Informa UK Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000238566500001
- Scopus ID
- 2-s2.0-33746867131
- Other Identifier
- 991014877784204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences
- Virology