Journal article
Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests
Biosensors (Basel), v 8(1)
26 Feb 2018
PMID: 29495424
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, with emphasis on minimally instrumented, point-of-care (POC) tests for resource-limited settings. Microfluidic cartridges ('chips') that combine solid-phase nucleic acid extraction; isothermal enzymatic nucleic acid amplification; pre-stored, paraffin-encapsulated lyophilized reagents; and real-time or endpoint optical detection are described. These chips can be used with a companion module for separating plasma from blood through a combined sedimentation-filtration effect. Three reporter types: Fluorescence, colorimetric dyes, and bioluminescence; and a new paradigm for end-point detection based on a diffusion-reaction column are compared. Multiplexing (parallel amplification and detection of multiple targets) is demonstrated. Low-cost detection and added functionality (data analysis, control, communication) can be realized using a cellphone platform with the chip. Some related and similar-purposed approaches by others are surveyed.
Metrics
Details
- Title
- Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests
- Creators
- Michael G. Mauk - Univ Penn, Sch Engn & Appl Sci, MEAM, Towne Bldg,220 33rd St, Philadelphia, PA 19104 USAJinzhao Song - University of PennsylvaniaChangchun Liu - University of PennsylvaniaHaim H. Bau - University of Pennsylvania
- Publication Details
- Biosensors (Basel), v 8(1)
- Publisher
- Mdpi
- Number of pages
- 30
- Grant note
- (R21) 1R21DE026700-01 / US National Institute of Health (NIH) National Institute of Dental and Craniofacial Research (NIDCR) Exploratory Development Grant (R21) 1R21AI128059-A1 / US National Institute of Health (NIH) National Institute of Allergies and Infectious Diseases (NIAID) Exploratory Development Grant
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000428551500016
- Scopus ID
- 2-s2.0-85043356202
- Other Identifier
- 991020623760204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical
- Instruments & Instrumentation
- Nanoscience & Nanotechnology