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Journal article
Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis
Frontiers in neuroscience, v 13, pp 665-665
03 Jul 2019
PMID: 31333398
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Drug-seeking in opioid dependence is due in part to the severe negative emotion associated with the withdrawal syndrome. It is well-established that negative emotional states emerge from activity in the amygdala. More recently, gut microflora have been shown to contribute substantially to such emotions. We measured gene expression in single glia and neurons gathered from the amygdala using laser capture microdissection and simultaneously measured gut microflora in morphine-dependent and withdrawn rats to investigate drivers of negative emotion in opioid withdrawal. We found that neuroinflammatory genes, notably
Tnf
, were upregulated in the withdrawal condition and that astrocytes, in particular, were highly active. We also observe a decreased
Firmicutes
to
Bacteroides
ratio in opioid withdrawal indicating gut dysbiosis. We speculate that these inflammatory and gut microflora changes contribute to the negative emotion experienced in opioid withdrawal that motivates dependence.
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Details
- Title
- Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis
- Creators
- Sean J. O’Sullivan - Thomas Jefferson UniversityEvangelia Malahias - Thomas Jefferson UniversityJames Park - Thomas Jefferson UniversityAnkita Srivastava - Thomas Jefferson UniversityBeverly A. S. Reyes - Drexel UniversityJon Gorky - , , , , , , , ,Rajanikanth Vadigepalli - Thomas Jefferson UniversityElisabeth J. Van Bockstaele - Drexel UniversityJames S. Schwaber - Thomas Jefferson University
- Publication Details
- Frontiers in neuroscience, v 13, pp 665-665
- Publisher
- Frontiers Media S.A
- Grant note
- National Institute on Drug Abuse U01 HL133360 / National Heart, Lung, and Blood Institute T32 AA007463 / National Institute on Alcohol Abuse and Alcoholism
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000473597100001
- Scopus ID
- 2-s2.0-85068431746
- Other Identifier
- 991019184825604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences