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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Single-molecule telomere length characterization by optical mapping in nano-channel array: Perspective and review on telomere length measurement
Environmental toxicology and pharmacology, v 82, 103562
Feb 2021
PMID: 33310082
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
•Telomere length can be used as a biomarker for aging, cancer, and DNA damage.•Telomere length characterization beyond average length measurement is necessary.•Our single molecule assay reveals new trends and features in aging, telomerase and ALT positive cancer cells.
In humans, the telomere consists of tandem 5′TTAGGG3′ DNA repeats on both ends of all 46 chromosomes. Telomere shortening has been linked to aging and age-related diseases. Similarly, telomere length changes have been associated with chemical exposure, molecular-level DNA damage, and tumor development. Telomere elongation has been associated to tumor development, caused due to chemical exposure and molecular-level DNA damage. The methods used to study these effects mostly rely on average telomere length as a biomarker. The mechanisms regulating subtelomere-specific and haplotype-specific telomere lengths in humans remain understudied and poorly understood, primarily because of technical limitations in obtaining these data for all chromosomes. Recent studies have shown that it is the short telomeres that are crucial in preserving chromosome stability. The identity and frequency of specific critically short telomeres potentially is a useful biomarker for studying aging, age-related diseases, and cancer. Here, we will briefly review the role of telomere length, its measurement, and our recent single-molecule telomere length measurement assay. With this assay, one can measure individual telomere lengths as well as identify their physically linked subtelomeric DNA. This assay can also positively detect telomere loss, characterize novel subtelomeric variants, haplotypes, and previously uncharacterized recombined subtelomeres. We will also discuss its applications in aging cells and cancer cells, highlighting the utility of the single molecule telomere length assay.
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Details
- Title
- Single-molecule telomere length characterization by optical mapping in nano-channel array: Perspective and review on telomere length measurement
- Creators
- Lahari Uppuluri - Drexel UniversityDharma Varapula - Drexel UniversityEleanor Young - Drexel UniversityHarold Riethman - Old Dominion UniversityMing Xiao - Drexel University
- Publication Details
- Environmental toxicology and pharmacology, v 82, 103562
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000615892500009
- Scopus ID
- 2-s2.0-85098200407
- Other Identifier
- 991019167624604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Environmental Sciences
- Pharmacology & Pharmacy
- Toxicology