Journal article
Sites and kinetics of donor thrombopoiesis following transplantation of whole bone marrow and progenitor subsets
Experimental hematology, v 35(10), pp 1567-1579
2007
PMID: 17697746
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Little is known about the sites and kinetics of thrombopoiesis following bone marrow transplant. The spleen is a site of hematopoiesis in a healthy mouse, and hematopoietic activity increases in response to stress. We hypothesized that the spleen is a major site of early post-transplant thrombopoiesis.
We transplanted whole bone marrow (WBM) or lineage depleted progenitor subsets fractionated based on expression of c-kit and Sca-1 from transgenic mice expressing green fluorescent protein into lethally irradiated C57BL/6 recipients. We also transplanted whole bone marrow cells into healthy and splenectomized mice. Post-transplant megakaryopoiesis was assessed by measuring circulating platelet number, percent donor-derived platelets, bone marrow cellularity, splenic weight, megakaryocyte size, and megakaryocyte concentration from hour 3 to day 28 post transplant.
Following transplant, circulating donor-derived platelets were derived only from c-kit expressing subsets. Donor-derived platelets first appeared on post-transplant day five. Splenectomy reduced the number of these earliest circulating platelets. Splenic megakaryopoiesis increased dramatically from day 7-14 post-transplant. However, splenectomy accelerated platelet engraftment during this time frame.
Overall, these results demonstrate that the first platelets are produced by c-kit expressing megakaryocyte progenitors in the bone marrow and spleen. After post-transplant day 5, the net effect of the spleen on thrombopoiesis is to slow engraftment due to immune effects or hypersplenism.
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Details
- Title
- Sites and kinetics of donor thrombopoiesis following transplantation of whole bone marrow and progenitor subsets
- Creators
- Xiao-Miao Li - Department of Pediatrics, University of Florida, Gainesville, Fla, USAZhongbo Hu - University of FloridaMartha Sola-Visner - Drexel UniversityStacey Hensel - University of FloridaRachel Garner - University of FloridaAbu-Bakr Zafar - University of FloridaJohn R. Wingard - University of FloridaMarda L. Jorgensen - University of FloridaRobert C. Fisher - University of FloridaEdward W. Scott - University of FloridaWilliam B. Slayton - University of FloridaXiaohua Hu - Information Science (Informatics)
- Publication Details
- Experimental hematology, v 35(10), pp 1567-1579
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Information Science
- Web of Science ID
- WOS:000250043100012
- Scopus ID
- 2-s2.0-34548673762
- Other Identifier
- 991019167662804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Hematology
- Medicine, Research & Experimental