Journal article
Size exclusion chromatography of synthetic polymers and biopolymers on common reversed phase and hydrophilic interaction chromatography columns
Journal of Chromatography A, v 1437, pp 74-87
11 Mar 2016
PMID: 26877177
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
•Common RP and HILIC columns can be used for SEC of synthetic polymers and biopolymers.•Solvation interactions determine polymer bulkiness and polymer-column steric interaction.•Solvation interactions depend on polymer, mobile phase and stationary phase polarities.•Column-mobile phase solvation interactions determine the structural order of the bonded ligands.•The stationary phase structural order affects packing pore volume and thus size-separation efficiency.•Medium polarity stationary phases can elute PS and PMMA polymers at the same retention volumes.
This work describes the applicability of common reversed phase and HILIC columns for size exclusion chromatography of synthetic and natural polymers. Depending on the nature of the solute and column stationary phase, a “non-retention” condition must be created with the aid of the mobile phase to achieve a unique size-based separation in isocratic mode. The various bonded phases show remarkable differences in size separations that are controlled by mobile phase conditions. Polymer-mobile phase and column-mobile phase solvation interactions determine polymer hydrodynamic volume (or solute bulkiness) and polymer-column steric interaction. Solvation interactions in turn depend on polymer, mobile phase and stationary phase polarities. Column-mobile phase solvation interactions determine the structural order of the bonded ligands that can vary from ordered (extended, aligned away from the silica substrate) to disordered (folded, pointing toward the silica substrate). Chain order increases with increased solvent penetration into the bonded phase. Increased chain order reduces pore volume, and therefore decreases the size-separation efficiency of a column. Conversely, decreased chain order increases pore volume and therefore increases the size-separation efficiency. The thermodynamic quality of the mobile phase also plays a significant role in the separation of polymers. “Poor” solvents can significantly reduce the hydrodynamic diameter of a solute and thus change their retention behavior. Medium polarity stationary phases, such as fluoro-phenyl and cyano, exhibit a unique retention behavior. With an appropriate polarity mobile phase, polar and non-polar synthetic polymers of the same molecular masses can be eluted at the same retention volumes.
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Details
- Title
- Size exclusion chromatography of synthetic polymers and biopolymers on common reversed phase and hydrophilic interaction chromatography columns
- Creators
- Anna M Caltabiano - Analytical Sciences and Development, GlaxoSmithKline, 1250 S. Collegeville Rd., Collegeville, PA 19426, USAJoe P Foley - Department of Chemistry, Drexel University, 3141Chestnut St., Philadelphia, PA 19104, USAHoward G Barth - Analytical Chemistry Consultants Ltd, 207 Welwyn Rd, Wilmington, DE 19803 USA
- Publication Details
- Journal of Chromatography A, v 1437, pp 74-87
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000371556700009
- Scopus ID
- 2-s2.0-84959204590
- Other Identifier
- 991014878500204721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical