Journal article
Size-exclusion chromatography using deuterated mobile phases
Journal of Chromatography A, v 1129(1), pp 41-46
2006
PMID: 16837004
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The effect of deuterated solvents in size-exclusion chromatography (SEC) was studied by comparing intrinsic viscosity measurements, SEC calibration curves, and column efficiency using water-soluble polymers. For aqueous SEC, the use of deuterium oxide slightly increases the SEC elution volume. To verify that adsorption onto the packing was absent, data from exclusion experiments were compared at 35 and 50
°C. Our results indicate that adsorption is not occurring for pullulan or polyethylene glycol (PEG)/poly(ethylene oxide) (PEO); for the latter, however, the elution volume increased using both D
2O and H
2O, indicative of slight hydrodynamic volume contraction of PEG/PEO at higher temperatures. A moderate increase in band broadening (moderate decrease in column efficiency) was observed using D
2O. Finally, the effects of chloroform versus deuterated chloroform were evaluated, but no hydrodynamic volume changes were observed.
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Details
- Title
- Size-exclusion chromatography using deuterated mobile phases
- Creators
- Jeanine M Erdner - Corporate Center for Analytical Sciences, DuPont Company, PO Box 80228, Wilmington, DE 19880-0228, USAHoward G Barth - Corporate Center for Analytical Sciences, DuPont Company, PO Box 80228, Wilmington, DE 19880-0228, USAJoe P Foley - Department of Chemistry, Drexel University, 3141 Chestnut St., Philadelphia, PA 19104, USAWilliam G Payne - Corporate Center for Analytical Sciences, DuPont Company, PO Box 80228, Wilmington, DE 19880-0228, USA
- Publication Details
- Journal of Chromatography A, v 1129(1), pp 41-46
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000240787600007
- Scopus ID
- 2-s2.0-33748296222
- Other Identifier
- 991014878039204721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical