Journal article
Size-selective uptake of colloidal low density lipoprotein aggregates by cultured white blood cells
Journal of colloid and interface science, v 350(2), pp 494-501
15 Oct 2010
PMID: 20667542
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This paper illustrates how principles of colloid science are useful in studying atherosclerosis. Accumulation of foam cells in the arterial intima is a key step in atherogenesis. The extent of foam cell formation is enhanced by low density lipoprotein (LDL) aggregates, and we have previously shown that the size of sphingomyelinase (Smase)-hydrolysis-induced aggregates depends directly on the concentration of ceramide generated in the LDL phospholipid monolayer, mediated by the hydrophobic effect. Here, we focus on the effect of LDL aggregate particle sizes on their subsequent uptake by macrophages. Our data show the first direct measurement of uptake as a function of aggregate size and the first direct comparison of uptake after Smase-catalyzed and vortex-mixing-mediated aggregation. Vortex-mixed aggregates with radii 20-77 nm showed maximal uptake approximately 118 microg sterol/mg protein at a 53 nm intermediate size, consistent with a mathematical model describing competition between aggregate surface area and volume. Smase-treated aggregates with radii 25-211 nm also showed maximal uptake at an intermediate size, approximately 58 microg sterol/mg protein for 132 nm particles, and fit a modified model that incorporated ceramide concentration expressed as aggregate size. This study shows that particle size is significant and composition may also be a factor in LDL uptake.
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Details
- Title
- Size-selective uptake of colloidal low density lipoprotein aggregates by cultured white blood cells
- Creators
- Michael J Walters - Drexel University, Department of Chemical and Biological Engineering, 3141 Chestnut Street, Philadelphia, PA 19104, USASteven P Wrenn
- Publication Details
- Journal of colloid and interface science, v 350(2), pp 494-501
- Publisher
- Elsevier; United States
- Grant note
- 5 R01 GM071355 / NIGMS NIH HHS R01 GM071355 / NIGMS NIH HHS R01 GM071355-05 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000281417800018
- Scopus ID
- 2-s2.0-77955773204
- Other Identifier
- 991014877839904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Physical