Journal article
Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype
The Journal of cell biology, v 213(3), pp 329-341
09 May 2016
PMID: 27138250
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Contemporary models for neuronal migration are grounded in the view that virtually all functionally relevant microtubules (MTs) in migrating neurons are attached to the centrosome, which occupies a position between the nucleus and a short leading process. It is assumed that MTs do not undergo independent movements but rather transduce forces that enable movements of the centrosome and nucleus. The present results demonstrate that although this is mostly true, a small fraction of the MTs are centrosome-unattached, and this permits limited sliding of MTs. When this sliding is pharmacologically inhibited, the leading process becomes shorter, migration of the neuron deviates from its normal path, and the MTs within the leading process become buckled. Partial depletion of ninein, a protein that attaches MTs to the centrosome, leads to greater numbers of centrosome-unattached MTs as well as greater sliding of MTs. Concomitantly, the soma becomes less mobile and the leading process acquires an elongated morphology akin to an axon.
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Details
- Title
- Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype
- Creators
- Anand N Rao - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129Aditi Falnikar - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129Eileen T O'Toole - Boulder Laboratory for 3D Electron Microscopy of Cells, University of Colorado, Boulder, CO 80309Mary K Morphew - Boulder Laboratory for 3D Electron Microscopy of Cells, University of Colorado, Boulder, CO 80309Andreas Hoenger - Boulder Laboratory for 3D Electron Microscopy of Cells, University of Colorado, Boulder, CO 80309Michael W Davidson - National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310 Department of Biological Science, Florida State University, Tallahassee, FL 32310Xiaobing Yuan - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129Peter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129 pbaas@drexelmed.edu
- Publication Details
- The Journal of cell biology, v 213(3), pp 329-341
- Publisher
- United States
- Grant note
- R56 NS028785 / NINDS NIH HHS F31 NS093748 / NINDS NIH HHS R01 NS028785 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000376144900008
- Scopus ID
- 2-s2.0-84983356924
- Other Identifier
- 991014878048104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology