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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Small molecule delivery across a perforated artificial membrane by thermoreversible hydrogel poloxamer 407
Colloids and surfaces, B, Biointerfaces, v 182, 110300
01 Oct 2019
PMID: 31326623
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
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•In saline, diffusion increases with total area of membrane perforations.•In a gel, diffusion does not scale with total area of membrane perforations.•In a gel, diffusion increases with a large perf but not with multiple small perfs.
Microperforations in the round window membrane have been suggested for enhancing the rate and reliability of drug delivery into the cochlea. Intratympanic injection, the most common delivery method, involves injecting therapy into the middle ear to establish a reservoir from which drug diffuses across the round window membrane into the cochlea. This process is highly variable because (i) the reservoir, if liquid, can lose contact with the membrane and (ii) diffusion across the membrane is intrinsically variable even with a stable reservoir. To address these respective sources of variability, we compared the thermoreversible hydrogel poloxamer 407 (P407) to saline as a drug carrier and studied the effect of membrane microperforations on drug diffusion rate. We used Rhodamine B as a drug proxy to measure permeance across an artificial membrane in a horizontal diffusion cell. We found that permeance of Rhodamine B from a saline reservoir was an order of magnitude higher than that from a P407 reservoir across unperforated membranes. Moreover, permeance increased with total perforation cross-sectional area regardless of number of perforations (p < 0.05 for all saline-based experiments), but the same association was not found with P407. Rather, for a P407 reservoir, only a large perforation increased permeance (p < 0.001), while multiple small perforations did not (p = 0.749). These results confirm that for drug dissolved in saline, multiple small perforations can effectively enhance diffusion. However, for drug dissolved in P407, larger perforations are necessary.
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Details
- Title
- Small molecule delivery across a perforated artificial membrane by thermoreversible hydrogel poloxamer 407
- Creators
- A. Santimetaneedol - Columbia UniversityZ. Wang - Columbia UniversityD.N. Arteaga - Columbia University Irving Medical CenterA. Aksit - Columbia UniversityC. Prevoteau - Columbia University Irving Medical CenterM. Yu - Columbia University Irving Medical CenterH. Chiang - Columbia University Irving Medical CenterD. Fafalis - Columbia UniversityA.K. Lalwani - Columbia UniversityJ.W. Kysar - Columbia University
- Publication Details
- Colloids and surfaces, B, Biointerfaces, v 182, 110300
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000489190200027
- Scopus ID
- 2-s2.0-85069000576
- Other Identifier
- 991021889995904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Chemistry, Physical
- Materials Science, Biomaterials