Journal article
Solid-state nanopore detection of protein complexes: applications in healthcare and protein kinetics
Small (Weinheim an der Bergstrasse, Germany), v 9(5), pp 750-759
11 Mar 2013
PMID: 23074081
Abstract
Protein conjugation provides a unique look into many biological phenomena and has been used for decades for molecular recognition purposes. In this study, the use of solid-state nanopores for the detection of gp120-associated complexes are investigated. They exhibit monovalent and multivalent binding to anti-gp120 antibody monomer and dimers. In order to investigate the feasibility of many practical applications related to nanopores, detection of specific protein complexes is attempted within a heterogeneous protein sample, and the role of voltage on complexed proteins is researched. It is found that the electric field within the pore can result in unbinding of a freely translocating protein complex within the transient event durations measured experimentally. The strong dependence of the unbinding time with voltage can be used to improve the detection capability of the nanopore system by adding an additional level of specificity that can be probed. These data provide a strong framework for future protein-specific detection schemes, which are shown to be feasible in the realm of a 'real-world' sample and an automated multidimensional method of detecting events.
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Details
- Title
- Solid-state nanopore detection of protein complexes: applications in healthcare and protein kinetics
- Creators
- Kevin J Freedman - Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, USAArangassery R BastianIrwin ChaikenMin Jun Kim
- Publication Details
- Small (Weinheim an der Bergstrasse, Germany), v 9(5), pp 750-759
- Publisher
- Wiley; Germany
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000315769700016
- Scopus ID
- 2-s2.0-84874752637
- Other Identifier
- 991014878170404721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter