Journal article
Solvent accessibility to flavin in oxynitilase
Biochimica et biophysica acta, Protein structure and molecular enzymology, v 830(1), pp 30-35
1985
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Oxynitrilase containing 2-thioFAD [C(2)=S]in place of FAD exhibits catalytic activity similar to that of native enzyme. Reaction of methyl metanethiolsulfonate with 2-thioFAD bound to oxynitrilase results in the formation of the corresponding flavin disulfide [C(2)-SSCH
3]. Normal flavin [C(2)=0] is formed by reacting 2-thioFAD oxynitrilase with
m-chloroperoxybenzoate or H
2O
2. Both reactions proceed via a spectrally detectable flavin 2-
S-oxide intermediate [
C(2)=
S
+-
O
−], but sizable amounts of this intermediate accumulate only in the
m-chloroperoxybenzoate reaction (about 40%). While similar reactions have been reported with free 2-thioflavin, kinetic and other data indicate that the oxynitrilase reactions occur with intact enzyme. This shows that the 2-position of the pyrimidine ring in the bound coenzyme is accessible to solvent. The data are consistent with previous studies on the reaction of peroxides with oxynitrilase-bound 5-deazaFAD which show that the pyrimidine ring is accessible at position 4. Analogous studies indicate that the pyrimidine ring is buried in the case of flavin bound to lactate oxidase, since the data indicate that both positions 2 and 4 are inaccessible to solvent.
Metrics
Details
- Title
- Solvent accessibility to flavin in oxynitilase
- Creators
- Marilyn Schuman Jorns - Hahnemann University Hospital
- Publication Details
- Biochimica et biophysica acta, Protein structure and molecular enzymology, v 830(1), pp 30-35
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1985ANL9100005
- Scopus ID
- 2-s2.0-0022428480
- Other Identifier
- 991019183937404721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics