Journal article
Solving nucleic acid structures by molecular replacement: examples from group II intron studies
Acta crystallographica. Section D, Structural biology, v 69(11), pp 2174-2185
Nov 2013
PMID: 24189228
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Structured RNA molecules are key players in ensuring cellular viability. It is now emerging that, like proteins, the functions of many nucleic acids are dictated by their tertiary folds. At the same time, the number of known crystal structures of nucleic acids is also increasing rapidly. In this context, molecular replacement will become an increasingly useful technique for phasing nucleic acid crystallographic data in the near future. Here, strategies to select, create and refine molecular-replacement search models for nucleic acids are discussed. Using examples taken primarily from research on group II introns, it is shown that nucleic acids are amenable to different and potentially more flexible and sophisticated molecular-replacement searches than proteins. These observations specifically aim to encourage future crystallographic studies on the newly discovered repertoire of noncoding transcripts.
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Details
- Title
- Solving nucleic acid structures by molecular replacement: examples from group II intron studies
- Creators
- Marco Marcia - Yale UniversityElisabeth Humphris-Narayanan - Yale UniversityKevin S. Keating - Yale UniversitySrinivas Somarowthu - Yale UniversityKanagalaghatta Rajashankar - Argonne National LaboratoryAnna Marie Pyle - Yale UniversityArgonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Publication Details
- Acta crystallographica. Section D, Structural biology, v 69(11), pp 2174-2185
- Publisher
- Int Union Crystallography
- Number of pages
- 12
- Grant note
- RO1GM50313 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 8 P41 GM103403-10; F32GM096516 / National Institute of General Medical Sciences of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) W-31-109-Eng-38 / US Department of Energy, Office of Science, Office of Basic Energy Sciences; United States Department of Energy (DOE)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000326648900003
- Scopus ID
- 2-s2.0-84887382371
- Other Identifier
- 991020837746404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Biophysics
- Crystallography