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Somatic Cell Encystment Promotes Abscission in Germline Stem Cells following a Regulated Block in Cytokinesis
Journal article   Open access   Peer reviewed

Somatic Cell Encystment Promotes Abscission in Germline Stem Cells following a Regulated Block in Cytokinesis

Kari F. Lenhart and Stephen DiNardo
Developmental cell, v 34(2), pp 192-205
27 Jul 2015
PMID: 26143993
url
http://www.cell.com/article/S1534580715003196/pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1016/j.devcel.2015.05.003View
Published, Version of Record (VoR) Open

Abstract

Cell Biology Developmental Biology Life Sciences & Biomedicine Science & Technology
In many tissues, the stem cell niche must coordinate behavior across multiple stem cell lineages. How this is achieved is largely unknown. We have identified delayed completion of cytokinesis in germline stem cells (GSCs) as a mechanism that regulates the production of stem cell daughters in the Drosophila testis. Through live imaging, we show that a secondary F-actin ring is formed through regulation of Cofilin activity to block cytokinesis progress after contractile ring disassembly. The duration of this block is controlled by Aurora B kinase. Additionally, we have identified a requirement for somatic cell encystment of the germline in promoting GSC abscission. We suggest that this non-autonomous role promotes coordination between stem cell lineages. These findings reveal the mechanisms by which cytokinesis is inhibited and reinitiated in GSCs and why such complex regulation exists within the stem cell niche.

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Web of Science research areas
Cell Biology
Developmental Biology
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