Journal article
Somatomedin-C/Insulinlike Growth Factor I (Sm-C/IGF-I) and Insulinlike Growth Factor II (IGF-II) mRNAs during Lung Development in the Rat
Experimental lung research, v 14(5), pp 607-618
1988
PMID: 2465142
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Somatomedins/insulinlike growth factors (Sm/IGFs) are peptide growth factors that are synthesized in multiple tissues and have been implicated in organ growth. Poly(A +)RNAsfrom lungs of fetal and postnatal rats were examined by dot and Northern blot analyses to investigate the local synthesis of IGFs during lung organogenesis. Blots were successively hybridized with 32P-labeled human IGF-II and mouse Sm-C/IGF-I cDNAs followed by human ubiquitin cDNAs (a control). Multiple IGF-II mRNA species of 4.7, 3.9, 3.1, 2.2, 1.75, and 1.2 kb were observed. Prenatally, the 3.9 kb species was predominant. In adult lung, the 4.7 kb species was the major species, and the 1.2 kb species was not detectable. Multiple Sm-C/IGF-I mRNA species of estimated sizes 7.5, 4.7, 1.7, and 1.0 kb were observed and their relative abundance did not change discernibly throughout development. Densitometric quantification revealed that IGF-II mRNAs were 7-20 fold more abundant in fetal lung than adult lung. In addition, they were 5-8 times more abundant than Sm-C/IGF-I mRNAs in embryonic lung, and were slightly less abundant than Sm-C/IGF-I mRNAs in adult lung. These data suggest synthesis of both Sm-C/IGF-I and IGF-II throughout lung organogenesis. Developmental changes in IGF-II mRNA abundance during lung organogenesis imply regulation at the level of gene transcription and/or mRNA stability.
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Details
- Title
- Somatomedin-C/Insulinlike Growth Factor I (Sm-C/IGF-I) and Insulinlike Growth Factor II (IGF-II) mRNAs during Lung Development in the Rat
- Creators
- Marsha L. Davenport - University of North Carolina at Chapel HillA. Joseph D'Ercole - University of North Carolina at Chapel HillJane C. Azizkhan - University of North Carolina at Chapel HillP. Kay Lund - 1From the Departments of Pediatrics and Physiology, University of North Carolina, Chapel Hill, NC, 27514
- Publication Details
- Experimental lung research, v 14(5), pp 607-618
- Publisher
- Informa UK Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1988Q281400004
- Scopus ID
- 2-s2.0-0023772142
- Other Identifier
- 991020200755004721
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- Web of Science research areas
- Respiratory System