Journal article
Spatial and temporal activation of the small GTPases RhoA and Rac1 by the netrin-1 receptor UNC5a during neurite outgrowth
Cellular signalling, v 21(12), pp 1961-1973
01 Dec 2009
PMID: 19755150
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Netrin-1 attracts or repels growing axons during development. The UNC5 receptors mediate the repulsive response, either alone or in complex with DCC receptors. The signaling mechanisms activated by UNC5 are poorly understood. Here, we examined the role of Rho GTPases in UNC5a signaling. We found that UNC5a induced neurite outgrowth in N1E-115 neuroblastoma cells in a netrin-1- and Rac1-dependent manner. UNC5a lacking its cytoplasmic tail also mediated this effect. In fibroblasts, UNC5a was able to activate RhoA and to a lower extent Rac1 and Cdc42 in response to netrin-1. Using Fluorescence Resonance Energy Transfer (FRET) intermolecular probes, we visualized the spatial and temporal activation of Rac1, Cdc42 and RhoA in live N1E-115 cells expressing UNC5a during neurite outgrowth. We found that Rac1 but not Cdc42 was transiently activated at the leading edge of the cell during neurite initiation. However, at later times when well-developed neurites were formed, active RhoA was found in the cell body and at the base of the neuronal leading process in UNC5a-expressing cells. Together, these findings demonstrate that the netrin-1 receptor UNC5a is able to induce neurite outgrowth and to differentially activate RhoA and Rac1 during neurite extension in a spatial and temporal manner. (C) 2009 Elsevier Inc. All rights reserved.
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Details
- Title
- Spatial and temporal activation of the small GTPases RhoA and Rac1 by the netrin-1 receptor UNC5a during neurite outgrowth
- Creators
- Marieve Picard - McGill UniversityRyan J. Petrie - McGill UniversityJudith Antoine-Bertrand - McGill UniversityEtienne Saint-Cyr-Proulx - McGill UniversityJosee-France Villemure - McGill UniversityNathalie Lamarche-Vane - McGill University
- Publication Details
- Cellular signalling, v 21(12), pp 1961-1973
- Publisher
- Elsevier
- Number of pages
- 13
- Grant note
- Fonds de la Recherche en Sante du Quebec (FRSQ); Fonds de la Recherche en Sante du Quebec MOP-14701 / Canadian Institute of Health Research (CIHR); Canadian Institutes of Health Research (CIHR)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000271269900026
- Scopus ID
- 2-s2.0-70349782280
- Other Identifier
- 991020099053004721
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- Web of Science research areas
- Cell Biology