Journal article
Specific In Situ Hepatitis B Viral Double Mutation (HBVDM) Detection in Urine with 60 copies/ml Analytical Sensitivity in a background of 250-fold Wild Type without DNA Isolation and Amplification
Analyst (London), v 140(5), pp 1590-1598
07 Mar 2015
PMID: 25599103
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have examined
in situ
detection of hepatitis B virus 1762T/1764A double mutation (HBVDM) in urine using a (Pb(Mg
1/3
Nb
2/3
)O
3
)
0.65
(PbTiO
3
)
0.35
(PMN-PT) piezoelectric plate sensor (PEPS) coated with a 16-nucleotide (nt) probe DNA (pDNA) complementary to the HBVDM. The
in situ
mutation (MT) detection was carried out in a flow with the PEPS vertically situated at the center of the flow in a background of the wild type (WT). For validation, the detection was followed with detection in the mixture of MT fluorescent reporter microspheres (FRMs) (MT FRMs) and WT FRMs that emitted different fluorescent colours and were designed to specifically bind to MT and WT, respectively. At 30°C and 4 ml/min, a PEPS was shown to specifically detect HBVDM
in situ
with 60 copies/ml analytical sensitivity in a background of clinically-relevant 250-fold more WT in 30 min without DNA isolation, amplification, or labelling as validated by the visualization of the captured MT FRMs and WT FRMs following the FRMs detection where the captured MT FRMs outnumbered WT FRMs by a factor of 5 to 1.
Metrics
Details
- Title
- Specific In Situ Hepatitis B Viral Double Mutation (HBVDM) Detection in Urine with 60 copies/ml Analytical Sensitivity in a background of 250-fold Wild Type without DNA Isolation and Amplification
- Creators
- Ceyhun E Kirimli - Drexel University, School of Biomedical Engineering, Science, and Health Systems, Philadelphia, Pennsylvania, USAWei-Heng Shih - Drexel University, Department of Materials Science and Engineering, Philadelphia, Pennsylvania, USAWan Y Shih - Drexel University, School of Biomedical Engineering, Science, and Health Systems, Philadelphia, Pennsylvania, USA
- Publication Details
- Analyst (London), v 140(5), pp 1590-1598
- Publisher
- Royal Society of Chemistry
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000349837200028
- Scopus ID
- 2-s2.0-84923169985
- Other Identifier
- 991014878116604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical