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Specificity of a target cell‐derived stop signal for afferent axonal growth
Journal article

Specificity of a target cell‐derived stop signal for afferent axonal growth

Douglas H Baird, Carlos A Baptista, Li‐Chong Wang and Carol A Mason
Journal of neurobiology, v 23(5), pp 579-591
Jul 1992
PMID: 1431837

Abstract

With a novel model culture system in which afferents are co‐cultured with purified populations of target neurons, we have demonstrated that a target cell within the central nervous system (CNS), the cerebellar granule neuron, poses a „stop‐growing signal”︁ for its appropriate afferents, the mossy fibers. To ask whether this stop signal is afferent specific, we co‐cultured granule neurons with an other cerebellar afferent system, the climbing fibers from the inferior olivary nuclei, which normally contact Purkinje neurons, and with retinal ganglion cell afferents, which never enter the cerebellum. Granule neurons do not pose a stop signal to either of these afferents. In contrast to pontine mossy afferents that grow well on laminin and showed reduced outgrowth on granule neurons, both olivary and retinal fibers displayed similar growth on laminin alone or on granule neurons. In addition, each afferent showed different degrees of fasciculation and growth cone morphology on laminin. Thus, the growth arrest signal sent by granule neurons is specifically recognized by their appropriate afferents. Moreover, these three types of afferents exhibit varying growth patterns on the same noncellular and cellular substrates, implicating distinct molecular characteristics of growth regulation for different classes of neurons that would contribute to specificity of synapse formation. © 1992 John Wiley & Sons, Inc.

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