Journal article
Specificity of cobamide remodeling, uptake and utilization in Vibrio cholerae
Molecular microbiology, v 113(1)
01 Jan 2020
PMID: 31609521
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cobamides are a group of compounds including vitamin B-12 that can vary at the lower base position of the nucleotide loop. They are synthesized de novo by only a subset of prokaryotes, but some organisms encode partial biosynthesis pathways for converting one variant to another (remodeling) or completing biosynthesis from an intermediate (corrinoid salvaging). Here, we explore the cobamide specificity in Vibrio cholerae through examination of three natural variants representing major cobamide groups: commercially available cobalamin, and isolated pseudocobalamin and p-cresolylcobamide. We show that BtuB, the outer membrane corrinoid transporter, mediates the uptake of all three variants and the intermediate cobinamide. Our previous work suggested that V. cholerae could convert pseudocobalamin produced by cyanobacteria into cobalamin. In this work, cobamide specificity in V. cholerae is demonstrated by remodeling of pseudocobalamin and salvaging of cobinamide to produce cobalamin. Cobamide remodeling in V. cholerae is distinct from the canonical pathway requiring amidohydrolase CbiZ, and heterologous expression of V. cholerae CobS was sufficient for remodeling. Furthermore, function of V. cholerae cobamide-dependent methionine synthase MetH was robustly supported by cobalamin and p-cresolylcobamide, but not pseudocobalamin. Notably, the inability of V. cholerae to produce and utilize pseudocobalamin contrasts with enteric bacteria like Salmonella.
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Details
- Title
- Specificity of cobamide remodeling, uptake and utilization in Vibrio cholerae
- Creators
- Amy T. Ma - Drexel UniversityBreanna Tyrell - Drexel UniversityJoris Beld - Drexel University
- Publication Details
- Molecular microbiology, v 113(1)
- Publisher
- Wiley
- Number of pages
- 14
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000493462800001
- Scopus ID
- 2-s2.0-85074852403
- Other Identifier
- 991019168286004721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Microbiology