Journal article
Spinal Cord Injury Reduces the Efficacy of Pseudorabies Virus Labeling of Sympathetic Preganglionic Neurons
Journal of neuropathology and experimental neurology, v 68(2), pp 168-178
01 Feb 2009
PMID: 19151624
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The retrograde transynaptic tracer pseudorabies virus (PRV) is used as a marker for synaptic connectivity in the spinal cord. Using PRV we sought to document putative synaptic plasticity below a high thoracic (T) spinal cord transection. This lesion has been linked to the development of a number of debilitating conditions including autonomic dysreflexia. Two weeks after injury, complete T4-transected and/or T4-hemisected and sham rats were injected with PRV-expressing enhanced green fluorescent protein (EGFP) or monomeric red fluorescent protein (mRFP1) into the kidneys. We expected greater PRV labeling after injury due to plasticity of spinal circuitry but 96 hours post-PRV-EGFP inoculation, we found fewer EGFP
+
cells in the thoracolumbar gray matter of T4-transected compared to sham rats (p < 0.01); Western blot analysis corroborated decreased EGFP protein levels (p < 0.01). Moreover, viral glycoproteins that are critical for cell adsorption and entry were also reduced in the thoracolumbar spinal cord of injured versus sham rats (p < 0.01). PRV labeling of sympathetic postganglionic neurons in the celiac ganglia innervating the kidneys was also significantly reduced in injured versus sham rats (p < 0.01). By contrast, the numbers and distribution of FluoroGold-labeled (intraperitoneal injection) sympathetic preganglionic neurons throughout the sampled regions appeared similar in injured and sham rats. These results question whether spinal cord injury exclusively retards PRV expression and/or transport or whether this injury broadly affects host cell-viral interactions.
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Details
- Title
- Spinal Cord Injury Reduces the Efficacy of Pseudorabies Virus Labeling of Sympathetic Preganglionic Neurons
- Creators
- Hanad Duale - University of KentuckyShaoping Hou - University of KentuckyAndrei V. Derbenev - University of KentuckyBret N. Smith - University of KentuckyAlexander G. Rabchevsky - University of Kentucky
- Publication Details
- Journal of neuropathology and experimental neurology, v 68(2), pp 168-178
- Publisher
- Lippincott
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; College of Medicine; Drexel University
- Web of Science ID
- WOS:000263012700006
- Scopus ID
- 2-s2.0-64049093784
- Other Identifier
- 991020099925304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Clinical Neurology
- Neurosciences
- Pathology