Logo image
Back
Spinal Dopaminergic Mechanisms Regulating the Micturition Reflex in Male Rats with Complete Spinal Cord Injury
Journal article   Open access   Peer reviewed

Spinal Dopaminergic Mechanisms Regulating the Micturition Reflex in Male Rats with Complete Spinal Cord Injury

Yuan Qiao, Zachary D Brodnik, Shunyi Zhao, Cameron T Trueblood, Zhenzhong Li, Veronica J Tom, Rodrigo A España and Shaoping Hou
Journal of neurotrauma, v 38(6), pp 803-817
15 Mar 2021
DOI: https://doi.org/10.1089/neu.2020.7284
PMID: 33297828
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1089/neu.2020.7284View
Published, Version of Record (VoR)Open Access (License Unspecified),  Open

Abstract

Animals Dopamine - metabolism Dopamine Antagonists - administration & dosage Electromyography - methods Female Male Rats Rats, Wistar Receptors, Dopamine D1 - antagonists & inhibitors Receptors, Dopamine D1 - metabolism Receptors, Dopamine D2 - agonists Receptors, Dopamine D2 - metabolism Spinal Cord Injuries - drug therapy Spinal Cord Injuries - metabolism Spinal Cord Injuries - physiopathology Thoracic Vertebrae - injuries Urinary Bladder - drug effects Urinary Bladder - metabolism Urinary Bladder - physiopathology Urination - drug effects Urination - physiology
Traumatic spinal cord injury (SCI) often causes micturition dysfunction. We recently discovered a low level of spinally-derived dopamine (DA) that regulates recovered bladder and sphincter reflexes in SCI female rats. Considering substantial sexual dimorphic features in the lower urinary tract, it is unknown if the DA-ergic mechanisms act in the male. Histological analysis showed a similar distribution of tyrosine hydroxylase (TH) neurons in the lower cord of male rats and the number increased following thoracic SCI. Subsequently, focal electrical stimulation in slices obtained from L6/S1 spinal segments of SCI rats elicited detectable DA release with fast scan cyclic voltammetry. Using bladder cystometrogram and external urethral sphincter (EUS) electromyography in SCI male rats, intravenous (i.v.) administration of SCH 23390, a D -like receptor (DR ) antagonist, induced significantly increased tonic EUS activity and a trend of increased residual volume, whereas activation of these receptors with SKF 38393 did not influence the reflex. Meanwhile, blocking spinal D -like receptors (DR ) with remoxipride had no effect but stimulating these receptors with quinpirole elicited EUS bursting to increase voiding volume. Further, intrathecal delivery of SCH 23390 and quinpirole resulted in similar responses to those with i.v. delivery, respectively, which indicates the central action regardless of delivery route. In addition, metabolic cage assays showed that quinpirole increased the voiding frequency and total voiding volume in spontaneous micturition. Collectively, spinal DA-ergic machinery regulates recovered micturition reflex following SCI in male rats; spinal DR tonically suppress tonic EUS activity to enable voiding and activation of DR facilitates voiding.

Metrics

11 Record Views
17 citations in Web of Science
15 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#5 Gender Equality

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Clinical Neurology
Critical Care Medicine
Neurosciences
Logo image