Journal article
Spontaneous respiratory plasticity following unilateral high cervical spinal cord injury in behaving rats
Experimental neurology, v 305, pp 56-65
01 Jul 2018
PMID: 29596845
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Unilateral cervical C2 hemisection (C2Hx) is a classic model of spinal cord injury (SCI) for studying respiratory dysfunction and plasticity. However, most previous studies were performed under anesthesia, which significantly alters respiratory network. Therefore, the goal of this work was to assess spontaneous diaphragm recovery post-C2Hx in awake, freely behaving animals. Adult rats were chronically implanted with diaphragm EMG electrodes and recorded during 8 weeks post-C2Hx. Our results reveal that ipsilateral diaphragm activity partially recovers within days post-injury and reaches pre-injury amplitude in a few weeks. However, the full extent of spontaneous ipsilateral recovery is significantly attenuated by anesthesia (ketamine/xylazine, isoflurane, and urethane). This suggests that the observed recovery may be attributed in part to activation of NMDA receptors which are suppressed by anesthesia. Despite spontaneous recovery in awake animals, ipsilateral hemidiaphragm dysfunction still persists: i) Inspiratory bursts during basal (slow) breathing exhibit an altered pattern, ii) the amplitude of sighs or augmented breaths is significantly decreased, and iii) the injured hemidiaphragm exhibits spontaneous events of hyperexcitation. The results from this study offer an under appreciated insight into spontaneous diaphragm activity and recovery following high cervical spinal cord injury in awake animals.
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Details
- Title
- Spontaneous respiratory plasticity following unilateral high cervical spinal cord injury in behaving rats
- Creators
- Tatiana Bezdudnaya - Drexel UniversityKristiina M. Hormigo - Drexel UniversityVitaliy Marchenko - Drexel UniversityMichael A. Lane - Drexel University
- Publication Details
- Experimental neurology, v 305, pp 56-65
- Publisher
- Elsevier
- Number of pages
- 10
- Grant note
- P01NS055976 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) SC140038 / United States Department of Defense (CDMRP) P01 NS 055976 / Spinal Cord Research Center at Drexel University, College of Medicine (NIH) Conquer Paralysis Now R01NS081112 / National Institute of Neurological Disorders And Stroke of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) Edward Jekkal Muscular Dystrophy Association
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Neurology
- Web of Science ID
- WOS:000433397500006
- Scopus ID
- 2-s2.0-85051603466
- Other Identifier
- 991019167344204721
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- Web of Science research areas
- Neurosciences